Sobiech K A, Karpiak S
Arch Immunol Ther Exp (Warsz). 1981;29(5):559-65.
After immunization of rabbits with a glycogen-protein complex from muscles of spurge hawk-moth (Celerio euphorbiae Linnaeus 1758), the immunoglobulin fraction was isolated from the obtained antiserum. The antibody has been found to reach in the immunodiffusion and immunoelectrophoretic tests with glycogen from philogenetically remote species. After the reaction with the immunoglobulin fraction glycogen preparations were less effectively degraded by alpha-amylase. The results suggest that the protein component is responsible for antigenicity of the glycogen-protein complex. The antibody was used to localize the complex in the muscles by the indirect immunofluorescence test.
用来自大戟天蛾（Celerio euphorbiae Linnaeus 1758）肌肉的糖原 - 蛋白质复合物免疫兔子后，从所得抗血清中分离出免疫球蛋白部分。已发现该抗体在与系统发育关系较远物种的糖原进行免疫扩散和免疫电泳试验时能产生反应。与免疫球蛋白部分反应后，糖原制剂被α - 淀粉酶降解的效率降低。结果表明蛋白质成分是糖原 - 蛋白质复合物抗原性的原因。通过间接免疫荧光试验，该抗体被用于在肌肉中定位该复合物。
