Miranda M, di Ilio C, Bonfigli A, Arcadi A, Pitari G, Dupre S, Federici G, del Boccio G
Biochim Biophys Acta. 1987 Jul 7;913(3):386-94. doi: 10.1016/0167-4838(87)90150-6.
The actions of glutathione S-transferase and tyrosinase on the in vitro production of glutathionyl-3,4-dihydroxyphenylalanine and the dopachrome level in the presence of GSH and L-3,4-dihydroxyphenylalanine were studied. No clear evidence of complementarity between tyrosinase and glutathione S-transferase was observed; on the contrary, in the presence of glutathione S-transferase the glutathionyl-3,4-dihydroxyphenylalanine yield was lower than with tyrosinase only, as measured by HPLC. It is concluded that the spontaneous conjugation of GSH with dopaquinone should probably be high enough to scavenge the toxic quinone and to produce precursors for phaeomelanogenesis.
研究了谷胱甘肽S-转移酶和酪氨酸酶在谷胱甘肽（GSH）和L-3,4-二羟基苯丙氨酸存在下对谷胱甘肽基-3,4-二羟基苯丙氨酸体外生成及多巴色素水平的作用。未观察到酪氨酸酶与谷胱甘肽S-转移酶之间存在明显互补性的证据；相反，通过高效液相色谱法测定，在谷胱甘肽S-转移酶存在下，谷胱甘肽基-3,4-二羟基苯丙氨酸的产量低于仅使用酪氨酸酶时的产量。得出的结论是，GSH与多巴醌的自发缀合可能足以清除有毒醌并产生褐黑素生成的前体。
