Solano-Muñoz F, Peñafiel R, Galindo J D
Biochem J. 1985 Aug 1;229(3):573-8. doi: 10.1042/bj2290573.
The pathway of dopachrome formation from L-dopa involves the net release of one proton for each molecule of dopachrome formed. The protons produced as a consequence of the enzymic step catalysed by tyrosinase can be measured by an electrometric device able to monitor changes in H+ concentration below 1 microM. This electrometric recording can be used as a simple, sensitive and continuous method for determining tyrosinase activity. The electrometric method can also be used in the presence of ascorbate by the spontaneous coupling of ascorbate oxidation to dopaquinone reduction, but measuring proton uptake instead of proton release.
从左旋多巴形成多巴色素的途径涉及到每形成一分子多巴色素净释放一个质子。由酪氨酸酶催化的酶促步骤产生的质子可以通过一种能够监测低于1微摩尔H⁺浓度变化的电位测定装置来测量。这种电位记录可以用作测定酪氨酸酶活性的一种简单、灵敏且连续的方法。通过抗坏血酸氧化与多巴醌还原的自发偶联，电位法也可以在抗坏血酸存在的情况下使用，但测量的是质子摄取而非质子释放。
