一种测定酪氨酸酶活性的电位测定法。
An electrometric method for the determination of tyrosinase activity.
作者信息
Solano-Muñoz F, Peñafiel R, Galindo J D
出版信息
Biochem J. 1985 Aug 1;229(3):573-8. doi: 10.1042/bj2290573.
Abstract
The pathway of dopachrome formation from L-dopa involves the net release of one proton for each molecule of dopachrome formed. The protons produced as a consequence of the enzymic step catalysed by tyrosinase can be measured by an electrometric device able to monitor changes in H+ concentration below 1 microM. This electrometric recording can be used as a simple, sensitive and continuous method for determining tyrosinase activity. The electrometric method can also be used in the presence of ascorbate by the spontaneous coupling of ascorbate oxidation to dopaquinone reduction, but measuring proton uptake instead of proton release.
摘要
从左旋多巴形成多巴色素的途径涉及到每形成一分子多巴色素净释放一个质子。由酪氨酸酶催化的酶促步骤产生的质子可以通过一种能够监测低于1微摩尔H⁺浓度变化的电位测定装置来测量。这种电位记录可以用作测定酪氨酸酶活性的一种简单、灵敏且连续的方法。通过抗坏血酸氧化与多巴醌还原的自发偶联，电位法也可以在抗坏血酸存在的情况下使用，但测量的是质子摄取而非质子释放。
相似文献
1
An electrometric method for the determination of tyrosinase activity.一种测定酪氨酸酶活性的电位测定法。
Biochem J. 1985 Aug 1;229(3):573-8. doi: 10.1042/bj2290573.
2
A kinetic study of the melanization pathway between L-tyrosine and dopachrome.L-酪氨酸与多巴色素之间黑色素化途径的动力学研究。
Biochim Biophys Acta. 1987 Feb 20;923(2):187-95. doi: 10.1016/0304-4165(87)90003-1.
3
A study on the in vitro interaction between tyrosinase and glutathione S-transferase.酪氨酸酶与谷胱甘肽S-转移酶体外相互作用的研究
Biochim Biophys Acta. 1987 Jul 7;913(3):386-94. doi: 10.1016/0167-4838(87)90150-6.
4
Dopachrome oxidoreductase: a new enzyme in the pigment pathway.多巴色素氧化还原酶：色素途径中的一种新酶。
J Invest Dermatol. 1984 Aug;83(2):145-9. doi: 10.1111/1523-1747.ep12263381.
5
Dopachrome conversion and dopa oxidase activities in recessive yellow mice. Catalytic activities of extracts from pheomelanic and eumelanic tissues.隐性黄色小鼠中的多巴色素转化和多巴氧化酶活性。从褐黑素和真黑素组织中提取的物质的催化活性。
J Hered. 1986 Sep-Oct;77(5):337-40. doi: 10.1093/oxfordjournals.jhered.a110251.
6
The role of pH in the melanin biosynthesis pathway.pH在黑色素生物合成途径中的作用。
J Biol Chem. 1982 Aug 10;257(15):8738-44.
7
Tyrosinase-catalyzed oxidation of dopa and related catechol(amine)s: a kinetic electron spin resonance investigation using spin-stabilization and spin label oximetry.酪氨酸酶催化多巴及相关儿茶酚（胺）的氧化：一项使用自旋稳定和自旋标记血氧测定法的动力学电子自旋共振研究
Biochim Biophys Acta. 1987 Jun 22;924(3):383-92. doi: 10.1016/0304-4165(87)90152-8.
8
Synergism exerted by 4-methyl catechol, catechol, and their respective quinones on the rate of DL-DOPA oxidation by mushroom tyrosinase.4-甲基邻苯二酚、邻苯二酚及其各自的醌对蘑菇酪氨酸酶催化DL-多巴氧化速率的协同作用。
Pigment Cell Res. 1992 Feb;5(1):41-8. doi: 10.1111/j.1600-0749.1992.tb00781.x.
9
Direct evidence for quinone-quinone methide tautomerism during tyrosinase catalyzed oxidation of 4-allylcatechol.酪氨酸酶催化4-烯丙基儿茶酚氧化过程中醌-醌甲基化物互变异构的直接证据。
Biochem Biophys Res Commun. 1995 Aug 15;213(2):469-74. doi: 10.1006/bbrc.1995.2155.
10
Inhibitory effects of melanin monomers, dihydroxyindole-2-carboxylic acid (DHICA) and dihydroxyindole (DHI) on mammalian tyrosinase, with a special reference to the role of DHICA/DHI ratio in melanogenesis.黑色素单体、二羟基吲哚 -2- 羧酸（DHICA）和二羟基吲哚（DHI）对哺乳动物酪氨酸酶的抑制作用，特别提及 DHICA/DHI 比值在黑色素生成中的作用。
Pigment Cell Res. 1995 Apr;8(2):105-12. doi: 10.1111/j.1600-0749.1995.tb00649.x.
引用本文的文献
1
Studies on enzymatic oxidation of 3',4'-dihydroxy-l-phenylalanine to dopachrome using kinetic isotope effect methods.使用动力学同位素效应方法对3',4'-二羟基-L-苯丙氨酸酶促氧化生成多巴色素的研究。
J Radioanal Nucl Chem. 2014;299(3):1373-1378. doi: 10.1007/s10967-013-2867-2. Epub 2013 Dec 10.
2
A new continuous spectrophotometric assay method for DOPA oxidase activity of tyrosinase.一种用于检测酪氨酸酶多巴氧化酶活性的新型连续分光光度测定法。
J Protein Chem. 2003 Jul;22(5):473-80. doi: 10.1023/b:jopc.0000005463.21302.cd.
3
Limitations of the quantitative cytochemical assay of catechol oxidase in melanoma cells.黑色素瘤细胞中儿茶酚氧化酶定量细胞化学测定法的局限性。
Histochem J. 1988 Nov;20(11):595-602. doi: 10.1007/BF01324077.
本文引用的文献
1
Biochemistry of melanin formation.黑色素形成的生物化学
Physiol Rev. 1950 Jan;30(1):91-126. doi: 10.1152/physrev.1950.30.1.91.
2
Separation, purification, and properties of two tyrosinases from hamster melanoma.从仓鼠黑色素瘤中分离、纯化两种酪氨酸酶及其性质
J Biol Chem. 1963 Jul;238:2351-7.
3
The isolation and properties of crystalline tyrosinase from Neurospora.来自脉孢菌的结晶酪氨酸酶的分离与特性
J Biol Chem. 1963 Jun;238:2045-53.
4
The role of pH in the melanin biosynthesis pathway.pH在黑色素生物合成途径中的作用。
J Biol Chem. 1982 Aug 10;257(15):8738-44.
5
An ultrasensitive electrometric system to measure membrane-bound acetylcholinesterase activity.一种用于测量膜结合乙酰胆碱酯酶活性的超灵敏测电系统。
Anal Biochem. 1983 Sep;133(2):302-6. doi: 10.1016/0003-2697(83)90088-x.
6
Pronounced formation of 5-OH-dopa at enzymatic oxidation of DOPA in the presence of ascorbic acid.在抗坏血酸存在的情况下，多巴酶促氧化时5-羟基多巴的显著形成。
Acta Derm Venereol. 1981;61(2):147-8.
7
A sensitive new assay for the oxidation of 3,4-dihydroxy L-phenylalanine by tyrosinase.一种用于检测酪氨酸酶氧化3,4-二羟基-L-苯丙氨酸的灵敏新测定法。
Anal Biochem. 1976 Sep;75(1):86-90. doi: 10.1016/0003-2697(76)90058-0.
Zotero 插件