State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2019 Sep;287:121399. doi: 10.1016/j.biortech.2019.121399. Epub 2019 Apr 30.
A high-solid loading pretreatment using NaOH/Urea solution at -12 °C was proposed to pretreat rice straw (RS) for enhanced saccharify and hydrogen production. Results shown NaOH/Urea pretreatment exhibited excellent pretreatment performance at solid loading ranged from 10% to 100% (w/v) with an average reducing sugar conversion of 80.22% was obtained which was 31.89% higher than that untreated RS. Upon fermentation of 100% solid loading pretreated hydrolysate, the H yield of 72.5 mL/g-pretreated RS was calculated based on substrate consumption, which enabled 49.5% higher reducing sugar transfer to H through material balance. FTIR and XRD analysis further demonstrated that the cold NaOH/Urea pretreatment at 100% (w/v) could effectively disrupt the lignin structure and decrease the cellulose crystallinity. The present study suggested a high solid loading pretreatment with NaOH/Urea at cold temperature could be a valuable alternative for better techno-economic of the lignocelluloses - to - sugars - to H routes.
采用 NaOH/Urea 溶液在-12°C 下对稻草进行高固含量预处理，以提高糖化和产氢效率。结果表明，NaOH/Urea 预处理在固含量为 10%至 100%（w/v）的范围内表现出优异的预处理性能，平均还原糖转化率为 80.22%，比未经处理的 RS 提高了 31.89%。在对 100%固含量预处理水解液进行发酵时，根据底物消耗计算出预处理 RS 的氢气产率为 72.5 mL/g，通过物料平衡将 49.5%的还原糖更有效地转化为氢气。FTIR 和 XRD 分析进一步表明，100%（w/v）的冷 NaOH/Urea 预处理可以有效破坏木质素结构并降低纤维素结晶度。本研究表明，在低温下采用高固含量 NaOH/Urea 预处理可以作为木质纤维素到糖到氢途径的更好技术经济替代方案。
