Akiyoshi T, Arinaga S, Tsuji H
Cancer Immunol Immunother. 1987;24(3):259-62. doi: 10.1007/BF00205640.
The effects of mitomycin C (MMC) on the generation of cell-mediated cytotoxicity in primary stimulation culture of human peripheral blood mononuclear cells (PBM) with the B lymphoblastoid Raji cell line were assessed. The cell-mediated cytotoxicity induced in culture was significantly augmented when MMC was added to cultures on day -1 to day 3 for 24 h at concentrations of 2.5 X 10(-2) micrograms/ml and 2.5 X 10(-3) micrograms/ml. To identify the cell populations affected by MMC, PBM were separated by adherence to plastic after treatment with MMC for 24 h (day -1). The two populations were recombined with untreated separated cells and stimulated with antigen. The ability to develop an augmented cell-mediated cytotoxicity was associated with the adherent cell fraction of MMC-treated PBM. Therefore, the ability of MMC-treated adherent cells to produce interleukin 1 (IL 1) was examined. Significantly higher levels of IL 1 were produced by treated cells as compared to untreated adherent cells. The results appear to indicate that the selective effects of MMC on the adherent cell fraction, especially the modification of IL 1 production, may be involved in the mechanisms of MMC-induced augmented cell-mediated cytotoxicity.
评估了丝裂霉素C（MMC）对人外周血单个核细胞（PBM）与B淋巴母细胞样Raji细胞系在初次刺激培养中细胞介导细胞毒性产生的影响。当在第-1天至第3天以2.5×10（-2）微克/毫升和2.5×10（-3）微克/毫升的浓度将MMC添加到培养物中24小时时，培养物中诱导的细胞介导细胞毒性显著增强。为了确定受MMC影响的细胞群体，在用MMC处理24小时（第-1天）后，通过贴壁于塑料上分离PBM。将这两个群体与未处理的分离细胞重新组合并用抗原刺激。增强的细胞介导细胞毒性的发展能力与MMC处理的PBM的贴壁细胞部分相关。因此，检测了MMC处理的贴壁细胞产生白细胞介素1（IL-1）的能力。与未处理的贴壁细胞相比，处理后的细胞产生的IL-1水平显著更高。结果似乎表明，MMC对贴壁细胞部分的选择性作用，特别是IL-1产生的改变，可能参与了MMC诱导的增强的细胞介导细胞毒性的机制。
