Verghese S, Curtis J, Turk J L
Cell Immunol. 1987 Jul;107(2):307-16. doi: 10.1016/0008-8749(87)90239-5.
Fluorescein isothiocyanate (FITC)-conjugated Mycobacterium leprae (FITC-M. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. At 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with FITC-M. leprae. At 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with haptenated M. leprae compared with those injected with unconjugated M. leprae. At 5 weeks, there was also a significant increase in delayed type hypersensitivity responses to 25 micrograms purified protein derivative. Histologically, epithelioid cell granulomas were seen in these lymph nodes as early as 2 weeks when FITC-M. leprae was used as the source of antigen. Enhancement in the immunogenicity of M. leprae by conjugation with FITC has been postulated.
将异硫氰酸荧光素（FITC）偶联的麻风分枝杆菌（FITC-M. leprae）皮内注射到豚鼠耳部，研究引流耳后淋巴结中的肉芽肿形成情况。在2周时，注射FITC-M. leprae的动物中有一半的引流淋巴结重量增加且有细胞浸润。在5周时，与注射未偶联麻风分枝杆菌的豚鼠相比，注射半抗原化麻风分枝杆菌的豚鼠的这些引流淋巴结重量和细胞浸润显著增加。在5周时，对25微克纯化蛋白衍生物的迟发型超敏反应也显著增加。组织学上，早在2周时，当使用FITC-M. leprae作为抗原来源时，这些淋巴结中就可见上皮样细胞肉芽肿。有人推测FITC与麻风分枝杆菌偶联可增强其免疫原性。
