从麻风分枝杆菌诱导的肉芽肿中分离出的细胞的辅助细胞功能。

Accessory cell function of cells isolated from Mycobacterium leprae-induced granulomas.

作者信息

Montreewasuwat N, Curtis J, Turk J L

出版信息

Cell Immunol. 1986 Oct 15;102(2):346-54. doi: 10.1016/0008-8749(86)90428-4.

Abstract

The large cells from Mycobacterium leprae-induced granulomas in guinea pig lymph nodes were separated by Percoll discontinuous density gradient centrifugation and on a fluorescence-activated cell sorter (FACS) using cross-reacting monoclonal antibody to human MHC Class II antigens. Large Percoll-separated cells (83% Class II antigen positive and 52% macrophage-specific antigen positive) and FACS-separated cells are able to act as antigen-presenting cells for T-cell proliferation to PPD. In previous studies, macrophage antigen-positive cells consistently failed to act as accessory cells. This indicates that there is a population of accessory cells which are macrophage antigen negative and MHC Class II antigen positive present in these M. leprae-induced granulomas.

摘要

利用针对人MHC II类抗原的交叉反应单克隆抗体，通过Percoll不连续密度梯度离心法，并在荧光激活细胞分选仪（FACS）上，分离出豚鼠淋巴结中麻风分枝杆菌诱导肉芽肿的大细胞。经Percoll分离的大细胞（83% II类抗原阳性，52%巨噬细胞特异性抗原阳性）和经FACS分离的细胞能够作为抗原呈递细胞，促进T细胞对结核菌素纯蛋白衍生物（PPD）的增殖。在先前的研究中，巨噬细胞抗原阳性细胞一直未能起到辅助细胞的作用。这表明在这些麻风分枝杆菌诱导的肉芽肿中存在一群辅助细胞，它们巨噬细胞抗原阴性但MHC II类抗原阳性。