Verghese S, Healey D G, Curtis J, Turk J L
Department of Pathology, Royal College of Surgeons of England, London.
Int Arch Allergy Appl Immunol. 1988;87(4):392-9. doi: 10.1159/000234708.
Dendritic cells were enriched from guinea pig auricular lymph nodes containing Mycobacterium leprae induced granulomas by immunomagnetic depletion of other cells. These cells were strongly positive for major histocompatibility complex class II antigens and labelled with an antidendritic cell monoclonal antibody, but not with an antimacrophage antibody. Interdigitating dendritic cells were identified in the granulomatous lymph node by staining with the antidendritic cell antibody and by transmission electron microscopy. When cultured in vitro with purified T lymphocytes, these cells acted as accessory cells for both purified protein derivative and concanavalin A induced proliferation. Although previous studies have shown that macrophages from these lymph nodes do not act as accessory cells, the present results indicate that dendritic cells from M. leprae granuloma containing lymph nodes may act as antigen-presenting cells.
通过对其他细胞进行免疫磁珠去除，从含有麻风分枝杆菌诱导肉芽肿的豚鼠耳淋巴结中富集树突状细胞。这些细胞对主要组织相容性复合体II类抗原呈强阳性，并用抗树突状细胞单克隆抗体标记，但不用抗巨噬细胞抗体标记。通过用抗树突状细胞抗体染色和透射电子显微镜在肉芽肿性淋巴结中鉴定出交错突树突状细胞。当与纯化的T淋巴细胞在体外培养时，这些细胞作为纯化蛋白衍生物和伴刀豆球蛋白A诱导增殖的辅助细胞。尽管先前的研究表明这些淋巴结中的巨噬细胞不作为辅助细胞，但目前的结果表明，来自含有麻风分枝杆菌肉芽肿的淋巴结中的树突状细胞可能作为抗原呈递细胞。
