Department of Microbiology, University of Washington, Seattle, WA, USA.
Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Sede Secondaria di Basovizza, Trieste, Italy.
Nat Commun. 2019 May 16;10(1):2183. doi: 10.1038/s41467-019-10201-4.
Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.
铜绿假单胞菌生物膜由胞外多糖 (EPS)、外源 DNA 和蛋白质组成，这些物质将这些群落结合在一起。铜绿假单胞菌产生凝集素 LecA 和 LecB，它们对基质 EPS 中发现的糖具有亲和力，并介导铜绿假单胞菌对靶宿主细胞的附着。在这里，我们证明 LecB 与 Psl 结合，Psl 是一种关键的基质 EPS，这导致细胞和 EPS 在不断生长的生物膜中的保留增加。这种相互作用预计会发生在凝集素和 Psl 上存在的分支侧链之间。最后，我们表明 LecB 协调生物膜中 Psl 的定位。这是 LecB 的一个独特功能，并将其鉴定为一种通过 EPS 相互作用有助于生物膜结构的基质蛋白。
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