Glukhova M A, Kabakov A E, Ornatsky O I, Vasilevskaya T D, Koteliansky V E, Smirnov V N
FEBS Lett. 1987 Jun 29;218(2):292-4. doi: 10.1016/0014-5793(87)81064-5.
150 kDa caldesmon was shown to be characteristic of vascular smooth muscle cells in normal tissue rather than in subculture. Subcultured smooth muscle cells from human aorta contained only the 70 kDa immunoreactive form of caldesmon. During the course of primary culture the amount of 150 kDa caldesmon as well as metavinculin decreased significantly whilst 70 kDa caldesmon became the predominant form, and by the onset of cell division the 150 kDa form was practically substituted by 70 kDa caldesmon. The data show that the predominance of 150 kDa caldesmon is characteristic of contractile smooth muscle cells, while in proliferating cells 70 kDa caldesmon is expressed.
150 kDa的钙调蛋白被证明是正常组织而非传代培养中的血管平滑肌细胞的特征。来自人主动脉的传代培养平滑肌细胞仅含有70 kDa免疫反应性形式的钙调蛋白。在原代培养过程中，150 kDa钙调蛋白以及间线蛋白的量显著减少，而70 kDa钙调蛋白成为主要形式，到细胞分裂开始时，150 kDa形式实际上被70 kDa钙调蛋白所取代。数据表明，150 kDa钙调蛋白占优势是收缩性平滑肌细胞的特征，而在增殖细胞中表达的是70 kDa钙调蛋白。
