Durand-Arczynska W, Marmy N, Durand J
Institute of Physiology, Fribourg, Switzerland.
Histochemistry. 1993 Dec;100(6):465-71. doi: 10.1007/BF00267827.
Calponin and caldesmon are two proteins considered to play a regulatory role in smooth muscle contraction, which have never previously been found to be expressed in subcultured cells. In the present study, immunocytochemistry and immunoblotting were performed to identify these proteins in smooth muscle cells (SMC) from human bronchi. It was found that human airway SMC, kept in a non-proliferative state, continued to express caldesmon and calponin at least until the 8th passage. The expression of alpha-smooth muscle actin studied under the same conditions was also shown to be preserved in subcultured bronchial SMC.
钙调蛋白和钙调素是两种被认为在平滑肌收缩中起调节作用的蛋白质，此前从未在传代培养细胞中发现它们的表达。在本研究中，采用免疫细胞化学和免疫印迹法在人支气管平滑肌细胞（SMC）中鉴定这些蛋白质。结果发现，处于非增殖状态的人气道SMC至少在第8代时仍持续表达钙调素和钙调蛋白。在相同条件下研究的α-平滑肌肌动蛋白的表达在传代培养的支气管SMC中也得以保留。
