Laboratory of Microbiology and Biochemistry, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, RS, Brazil.
Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, RS, Brazil.
Bioresour Technol. 2019 Sep;287:121406. doi: 10.1016/j.biortech.2019.121406. Epub 2019 May 2.
The objective of this study was to evaluate the biofixation and production of biocompounds by Chlorella fusca LEB 111 cultivated with different concentrations of carbon dioxide (CO) adsorbent nanofibers in their free form or retained. Cultures were grown in 15% (v v) CO with 0.1, 0.3 and 0.5 g L nanofibers developed with 10% (w v) polyacrylonitrile (PAN)/dimethylformamide (DMF), with or without nanoparticles; retained or not. The addition of 0.1 g L nanofibers with nanoparticles in their free form to the cultures promoted the accumulation of approximately 3 times more carbon in the medium (46.6 mg L), a 45% higher biofixation rate (89.2 mg L d) and increased carbohydrate production by approximately 2.3% (w w) of that observed in cultures grown without nanofibers. Therefore, nanofibers showed promising potential as physical adsorbents of CO in the cultivation to increase gas fixation and promote the synthesis of macromolecules.
本研究的目的是评估游离或保留不同浓度二氧化碳（CO）吸附纳米纤维的小球藻 LEB 111 的生物固定和生物化合物的产生。在 15%（v/v）CO 中培养，使用 10%（w/v）聚丙烯腈（PAN）/二甲基甲酰胺（DMF）开发的 0.1、0.3 和 0.5 g/L 纳米纤维，添加或不添加纳米颗粒；保留或不保留。将游离形式的含有纳米颗粒的 0.1 g/L 纳米纤维添加到培养物中，促进了培养基中约 3 倍更多的碳积累（46.6 mg/L），生物固定率提高了 45%（89.2 mg/L/d），碳水化合物产量增加了约 2.3%（与不添加纳米纤维的培养物相比）。因此，纳米纤维作为 CO 的物理吸附剂在培养中具有很大的潜力，可增加气体固定并促进大分子的合成。
