E74 样转录因子 3（ELF3）是膀胱癌上皮-间充质转化的负调控因子。
E74 like ETS transcription factor 3 (ELF3) is a negative regulator of epithelial- mesenchymal transition in bladder carcinoma.
机构信息
Institute of Bioinformatics, International Technology Park, Bangalore, India.
Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, India.
出版信息
Cancer Biomark. 2019;25(2):223-232. doi: 10.3233/CBM-190013.
BACKGROUND
Transcription factors are commonly deregulated in various cancers. Here, we evaluated role of ELF3 in pathogenesis of bladder carcinoma (BCa).
MATERIALS AND METHODS
We confirmed ELF3 expression pattern in BCa cell lines using western blot; and in different grades of tumors using Immunohistochemistry. Cell invasion assay was employed to demonstrate potential role of ELF3 in EMT.
RESULTS AND CONCLUSION
ELF3 showed selective expression in low-grade cell lines and tumor tissues. Overexpression of ELF3 in mesenchymal cell line UMUC3 resulted in reduced invasion and decreased expression of mesenchymal markers. We observed association of low ELF3 expression with increased risk and overall poor survival using publicly available data. ELF3-modulated reversal of EMT might be a useful strategy in the treatment of bladder cancer.
背景
转录因子在各种癌症中通常失调。在这里，我们评估了 ELF3 在膀胱癌（BCa）发病机制中的作用。
材料和方法
我们使用 Western blot 验证了 ELF3 在 BCa 细胞系中的表达模式；并使用免疫组织化学验证了不同分级肿瘤中的表达。细胞侵袭实验用于证明 ELF3 在 EMT 中的潜在作用。
结果和结论
ELF3 在低级别细胞系和肿瘤组织中表现出选择性表达。ELF3 在间充质细胞系 UMUC3 中的过表达导致侵袭减少和间充质标志物表达降低。我们使用公开数据观察到低 ELF3 表达与风险增加和总体预后不良有关。ELF3 调节的 EMT 逆转可能是治疗膀胱癌的一种有用策略。
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