College of Agriculture, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu 210095, PR China; and College of Agriculture, Yangzhou University, Yangzhou 225009, PR China.
College of Agriculture, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu 210095, PR China.
Funct Plant Biol. 2019 Jul;46(8):715-724. doi: 10.1071/FP18192.
Soil waterlogging and high temperature conditions generally occur together, especially in the Yangtze River Valley, China, negatively affecting cotton (Gossypium hirsutum L.) fibre development. Therefore, combined elevated temperature (34.1/29.0°C) and soil waterlogging (6 days) were imposed to study their combined effects on fibre biomass and fibre qualities (length, strength and micronaire). The results showed that in the boll cohort exposed to waterlogging and/or elevated air temperature, combined elevated temperature and soil waterlogging decreased final fibre length (by 8.9-11.3%) and fibre biomass (by 25.8-33.9%) more than either stress individually. A total of 113, 263 and 290 differential abundance proteins were identified related to elevated temperature, waterlogging and the two treatments combined, respectively, in fibres at 15 days after anthesis via the isobaric tags for relative and absolute quantitation technique, which were classified as: carbohydrate and energy metabolism (21.7%), protein metabolism (16.6%), amino acid metabolism (12.8%), intracellular structural components (6.6%), transport (7.9%), oxidation-reduction process (7.9%), signal transduction (5.2%), lipid metabolism (5.2%), stress response (5.2%), nucleic acid metabolism (4.5%), organic acid metabolism (3.4%) and others (2.1%). Both vacuolar ATPase (V-ATPase) and plasma membrane H+-ATPase (PMH+-ATPase) were responsible for fibre length formation, although V-ATPase expression may play a major role in determining fibre cell elongation rather than PM H+-ATPase expression. It was concluded that fibre cell elongation and secondary wall thickening were inhibited mainly by reduced accumulation of osmolytes, blocked synthesis and transport of secondary wall components, and disruption of the cytoskeleton system under combined elevated temperature and soil waterlogging.
土壤渍水和高温条件通常同时出现，特别是在中国长江流域，对棉花（Gossypium hirsutum L.）纤维发育产生负面影响。因此，本研究模拟了同时升高温度（34.1/29.0°C）和土壤渍水（6 天）的环境，以研究它们对纤维生物量和纤维品质（长度、强度和马克隆值）的综合影响。结果表明，在暴露于渍水和/或升高空气温度的棉铃群体中，与单独的任一胁迫相比，升高温度和土壤渍水的综合处理使最终纤维长度（减少 8.9-11.3%）和纤维生物量（减少 25.8-33.9%）减少更多。通过相对和绝对定量同位素标记技术，在开花后 15 天的纤维中，分别鉴定出与升高温度、渍水和两种处理组合相关的 113、263 和 290 个差异丰度蛋白，这些蛋白被归类为：碳水化合物和能量代谢（21.7%）、蛋白质代谢（16.6%）、氨基酸代谢（12.8%）、细胞内结构成分（6.6%）、运输（7.9%）、氧化还原过程（7.9%）、信号转导（5.2%）、脂质代谢（5.2%）、应激反应（5.2%）、核酸代谢（4.5%）、有机酸代谢（3.4%）和其他（2.1%）。液泡型 ATP 酶（V-ATPase）和质膜 H+-ATP 酶（PM H+-ATPase）都负责纤维长度的形成，尽管 V-ATPase 表达可能在决定纤维细胞伸长方面起主要作用，而不是 PM H+-ATPase 表达。研究结论认为，在综合升高温度和土壤渍水条件下，纤维细胞伸长和次生壁增厚受到抑制，主要是由于渗透物积累减少、次生壁成分的合成和运输受阻以及细胞骨架系统紊乱。
