Lau D C, Wong K L, Tough S C
Metabolism. 1987 Jul;36(7):631-6. doi: 10.1016/0026-0495(87)90145-4.
Study of the vascular endothelium has been greatly facilitated by the development of specific cell culture systems isolated from various tissues. We report herein a simple method for establishing a propagating cell culture system of microvascular endothelial cells derived from rat adipose tissue. In addition to the characteristic cobblestone appearance on light microscopy, the microvascular endothelial cells in culture also demonstrated the presence of other markers for large vessel endothelia. Electron microscopy revealed endothelium-specific Weibel-Palade bodies and abundant pinocytotic vesicles. Both retroperitoneal and epididymal endothelia demonstrated the presence of factor VIII antigen by immunofluorescent staining and prostacyclin production. Although there was no appreciable morphological difference between cultured retroperitoneal and epididymal microvascular endothelia, the replication rate of the former was significantly higher than that of the latter (P less than .05). Excessive replication of endothelial cells may play a role in the regional differences of adipose tissue mass in an organism.
从各种组织中分离出的特定细胞培养系统的发展极大地推动了对血管内皮的研究。我们在此报告一种简单的方法，用于建立源自大鼠脂肪组织的微血管内皮细胞的增殖细胞培养系统。除了在光学显微镜下呈现出特征性的鹅卵石样外观外，培养的微血管内皮细胞还显示出存在其他大血管内皮细胞的标志物。电子显微镜显示有内皮特异性的魏-帕小体和丰富的吞饮小泡。腹膜后和附睾内皮通过免疫荧光染色显示存在因子VIII抗原并能产生前列环素。尽管培养的腹膜后和附睾微血管内皮在形态上没有明显差异，但前者的复制率明显高于后者（P小于0.05）。内皮细胞的过度复制可能在生物体脂肪组织量的区域差异中起作用。
