Okabayashi T
Nihon Geka Gakkai Zasshi. 1987 May;88(5):529-42.
Immunohistochemical study with various monoclonal antibodies against the mononuclear cell surface antigen was performed on the regional lymph nodes of gastric cancer through Avidin-Biotin Complex (ABC) method. In the paracortical area (P.C.) of those lymph nodes without the metastasis of gastric cancer, T cells were dominant, and OKT3 positive (OKT3+) cells and OKT4+ cells were diffusely present, while OKT8+ cells were occasionally recognized. In the sinus, subsets of the above T cells, and OKT9+ cells and OKT10+ cells were observed. In the germinal center (G.C.), mantle zone (M.Z.) and primary follicle (P.F.), which were B cell regions, OKIa1+ cells and Leu12+ cells were diffusely present. OKB7+ cells, OKT9+ cells, OKT10+ cells and Leu7+ cells were also noted in G.C.. Leu8+ cells were observed in M.Z. and P.F.. OKIa1+ cells were occasionally noted in P.C. and sinus. In the lymph nodes with the metastasis, decrease of OKT4+ cells and increase of OKT8+ cells were noted in comparison to the lymph nodes without the metastasis. Using the tissue double fluorescence staining method, it was found that about half of the OKT4+ cells were helper T cells. The majority of OKT8+ cells were identified as cytotoxic T cells or their precursors. By preoperative endoscopic administration of OK-432 or PSK into the tumor, the IL-2 receptor+ cells, or OKM1+ cells and OKT4+ cells increased in the regional lymph nodes and the antitumor activity was intensified.
采用抗单核细胞表面抗原的多种单克隆抗体，通过抗生物素蛋白-生物素复合物（ABC）法对胃癌区域淋巴结进行免疫组织化学研究。在无胃癌转移的淋巴结副皮质区（P.C.），T细胞占主导，OKT3阳性（OKT3+）细胞和OKT4+细胞弥漫性存在，而OKT8+细胞偶尔可见。在窦区，可观察到上述T细胞亚群以及OKT9+细胞和OKT10+细胞。在生发中心（G.C.）、套区（M.Z.）和初级滤泡（P.F.）这些B细胞区域，OKIa1+细胞和Leu12+细胞弥漫性存在。在G.C.中也可见OKB7+细胞、OKT9+细胞、OKT10+细胞和Leu7+细胞。在M.Z.和P.F.中观察到Leu8+细胞。在P.C.和窦区偶尔可见OKIa1+细胞。在有转移的淋巴结中，与无转移的淋巴结相比，OKT4+细胞减少，OKT8+细胞增加。采用组织双重荧光染色法发现，约一半的OKT4+细胞为辅助性T细胞。大多数OKT8+细胞被鉴定为细胞毒性T细胞或其前体。术前通过内镜向肿瘤内注射OK-432或PSK后，区域淋巴结中的IL-2受体+细胞、OKM1+细胞和OKT4+细胞增加，抗肿瘤活性增强。
