Hiramatsu S
First Department of Surgery, Okayama University Medical School, Japan.
Nihon Geka Gakkai Zasshi. 1987 Nov;88(11):1591-603.
Immunohistochemical study was performed on the spleens of the stomach cancers using various monoclonal antibodies against mononuclear cells. The subjects consisted of 4 cases in stage I, 3 cases in stage II, 9 cases in stage III and 15 cases in stage IV. Five spleens of patients died of cerebrovascular disorders were used as a control. For 6 among these cancer cases, PSK was injected endoscopically into the tumor 10 days before operation. The results showed that: 1) OKT3+, OKT4+ and OKT8+ positive cells decreased in the marginal area of follicles with the progression of the stage of stomach cancer. 2) OKM1 and OKT10 positive cells increased in the marginal area of follicles and the red pulp of spleens which were in the latter stage of the stomach cancer. 3) OKIa1+ and OKB7+ positive cells decreased in the follicles in the later stage. 4) Leu7+, Leu11b+, OKT9+ and IL2 receptor+ cells apparently did not change in spite of the progression of the stage of stomach cancer. 5) OKT6+ positive cells were hardly noted in the spleen. 6) In PSK injected group, OKT4+, OKT8+ and OKB7+ cells tended to increase, while IL2 receptor+ cells and OKM1+ cells were definitely observed to increase. In conclusion, the spleen of the stomach cancer in the advanced stages exhibited the decrease of the T-cell system such as OKT3+, OKT4+ and OKT8+ cells and it is believed that the immune response was lowered through T-cell system as the stage advances. At the same time, the fact of a similar decrease in the B-cell system of OKB7+ seems to suggest a lowered state of antibody productivity. On the other hand, the increase of OKM1+ and OKT10+ cells in the advanced stage is considered to be a state of activation in the reticuloendothelial system. In the PSK injected cases, the increase of OKT4+, OKT8+ and IL2 receptor+ cells was observed. This was suggested that intratumorous injection of PSK was able to enhance the antitumor activity through the spleen by the activation of the T-cell system and reticuloendothelial system.
使用多种抗单核细胞的单克隆抗体，对胃癌患者的脾脏进行免疫组织化学研究。研究对象包括I期4例、II期3例、III期9例和IV期15例。将5例死于脑血管疾病患者的脾脏用作对照。在这些癌症病例中，有6例在手术前10天通过内镜将PSK注入肿瘤。结果显示：1）随着胃癌分期的进展，卵泡边缘区域的OKT3 +、OKT4 +和OKT8 +阳性细胞减少。2）在胃癌后期的脾脏卵泡边缘区域和红髓中，OKM1和OKT10阳性细胞增加。3）后期卵泡中OKIa1 +和OKB7 +阳性细胞减少。4）尽管胃癌分期进展，但Leu7 +、Leu11b +、OKT9 +和IL2受体 +细胞明显没有变化。5）在脾脏中几乎未观察到OKT6 +阳性细胞。6）在PSK注射组中，OKT4 +、OKT8 +和OKB7 +细胞有增加趋势，而IL2受体 +细胞和OKM1 +细胞确实观察到增加。总之，晚期胃癌患者的脾脏表现出OKT3 +、OKT4 +和OKT8 +等T细胞系统减少，并且认为随着分期进展，免疫反应通过T细胞系统降低。同时，OKB7 +的B细胞系统出现类似减少这一事实似乎表明抗体产生能力降低。另一方面，晚期OKM1 +和OKT10 +细胞的增加被认为是网状内皮系统的激活状态。在PSK注射病例中，观察到OKT4 +、OKT8 +和IL2受体 +细胞增加。这表明肿瘤内注射PSK能够通过激活T细胞系统和网状内皮系统增强脾脏的抗肿瘤活性。
