Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil.
Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil.
J Biomed Mater Res B Appl Biomater. 2020 Apr;108(3):663-673. doi: 10.1002/jbm.b.34420. Epub 2019 May 20.
This study aimed to investigate in vitro and in vivo the influence of hyperglycemic condition on biocompatibility and biomineralization of gray mineral trioxide aggregate (GMTA) and white mineral trioxide aggregate (WMTA). For the in vitro study, fibroblast-like cells L929 were cultured under high or normal glucose concentration to investigate the effects of both MTA's on cell proliferation and inflammatory cytokines production IL-1β, IL-6, and TNF-α. For the in vivo study, polyethylene tubes containing MTA materials and empty tubes were implanted into dorsal connective tissues of Wistar rats previously assigned normal and hyperglycemic. After 7 and 30 days, the tubes with surrounding tissues were removed and subjected to histological, fluorescence and immunohistochemical analyzes of IL-1β, IL-6, and TNF-α. In vitro study showed that, under high glucose condition, GMTA reduced cell proliferation and IL-6 production compared with WMTA. Moreover, in vivo study revealed that hyperglycemic condition did not modify the inflammatory response and cytokines production in the tissue close to both materials. Independently of hyperglycemic status, mineralized areas were observed with both materials, but the fluorescence intensity of WMTA was diminished on 14 days in hyperglycemic animals. It is possible to conclude that GMTA was able to inhibit the proliferation rate and IL-6 production under high glucose concentration in vitro. Furthermore, cytokines production and inflammatory response were not upregulated in hyperglycemic animals; however, a decrease in the calcium deposition was observed in presence of WMTA, suggesting a delay in the mineralization process.
本研究旨在体外和体内研究高血糖条件对灰色矿化三氧化物凝聚体（GMTA）和白色矿化三氧化物凝聚体（WMTA）的生物相容性和生物矿化的影响。在体外研究中，将成纤维样细胞 L929 在高糖或正常葡萄糖浓度下培养，以研究两种 MTA 对细胞增殖和炎性细胞因子（IL-1β、IL-6 和 TNF-α）产生的影响。在体内研究中，将含有 MTA 材料的聚乙烯管和空管植入先前分配为正常和高血糖的 Wistar 大鼠的背部结缔组织中。7 天和 30 天后，取出带有周围组织的管子，并进行组织学、荧光和免疫组织化学分析，以检测 IL-1β、IL-6 和 TNF-α。体外研究表明，在高糖条件下，GMTA 与 WMTA 相比，降低了细胞增殖和 IL-6 的产生。此外，体内研究表明，高血糖状态并没有改变两种材料附近组织的炎症反应和细胞因子的产生。无论高血糖状态如何，两种材料都观察到有矿化区，但在高血糖动物中，WMTA 的荧光强度在 14 天减弱。可以得出结论，GMTA 能够抑制体外高糖浓度下的细胞增殖率和 IL-6 的产生。此外，高血糖动物的细胞因子产生和炎症反应没有上调；然而，在 WMTA 存在下观察到钙沉积减少，表明矿化过程延迟。
