Takesawa S, Ohmi S, Konno Y, Sekiguchi M, Shitaokoshi S, Takahashi T, Hidai H, Sakai K
Nephrol Dial Transplant. 1987;1(4):254-7.
This study elucidates changes in membrane structure and permeability due to the methods of sterilisation and the conditions under which they are carried out. Tubular dialysis membranes of regenerated cellulose having various values for porosity were sterilised by ethylene oxide gas, autoclave or gamma irradiation under varying conditions. Non-sterilised membranes were included as controls. The solute permeability of the membranes was determined using 14C-urea. The membranes tested showed no difference in clearance of urea or creatinine. Gamma-ray sterilisation under dry conditions greatly reduced the vitamin B12 clearance and hydraulic permeability of membranes with a water content of below 60%. Hydraulic permeability increased with gamma irradiation for membranes sterilised under wet conditions. A reduction in vitamin B12 clearance for membranes with a water content of above 60% resulted after autoclave sterilisation. Pore model calculation reveals that membrane shrinkage resulted from sterilisation both by gamma-rays under dry conditions, and by autoclave. Thus, the structure of dialysis membranes varies with the method of sterilisation and the conditions under which the sterilisation is carried out.
本研究阐明了由于灭菌方法及其实施条件而导致的膜结构和通透性的变化。对具有不同孔隙率值的再生纤维素管状透析膜，在不同条件下用环氧乙烷气体、高压灭菌器或伽马射线进行灭菌处理。未灭菌的膜作为对照。使用14C-尿素测定膜的溶质通透性。所测试的膜在尿素或肌酐清除率方面没有差异。在干燥条件下进行伽马射线灭菌，极大地降低了含水量低于60%的膜的维生素B12清除率和水力通透性。对于在潮湿条件下灭菌的膜，水力通透性随伽马射线照射而增加。在高压灭菌后，含水量高于60%的膜的维生素B12清除率降低。孔隙模型计算表明，干燥条件下的伽马射线灭菌和高压灭菌均会导致膜收缩。因此，透析膜的结构随灭菌方法及其实施条件而变化。
