Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, China.
Department of Animal and Veterinary Sciences, University of Vermont, Burlington, Vermont.
J Anim Physiol Anim Nutr (Berl). 2019 Sep;103(5):1610-1618. doi: 10.1111/jpn.13126. Epub 2019 May 20.
This study was to compare the effects of parenteral supplementation of methionyl-methionine (Met-Met) or Met on intestinal barrier function in Met-deficient pregnant mice. Pregnant mice were randomly divided into three groups. The Control group was provided a diet containing Met and received i.p. injection of saline. The Met group was fed the same diet but without Met and received daily i.p. injection of 35% of the Met contained in the control diet. The Met-Met group was treated the same as the Met group, except that 25% of the Met injected was replaced with Met-Met. Met-Met promoted villus surface area in ileum compared with Met alone. In addition, the mRNA abundance of amino acid and glucose transporters in the small intestine was altered with Met-Met. Moreover, Met-Met increased tight junction protein and decreased apoptosis-related proteins expression in the jejunum and ileum. These results suggest that Met-Met can promote intestinal function over Met alone in Met-deficient mice.
本研究旨在比较蛋氨酸-蛋氨酸（Met-Met）或蛋氨酸（Met）肠外补充对蛋氨酸缺乏的妊娠小鼠肠屏障功能的影响。将妊娠小鼠随机分为三组。对照组给予含蛋氨酸的饮食，并接受腹腔注射生理盐水。Met 组给予相同的饮食，但不含蛋氨酸，并每天腹腔注射相当于对照组饮食中蛋氨酸含量的 35%。Met-Met 组与 Met 组的治疗方法相同，只是注射的蛋氨酸中有 25%被 Met-Met 替代。与单独使用 Met 相比，Met-Met 可促进回肠绒毛表面积。此外，Met-Met 改变了小肠中氨基酸和葡萄糖转运体的 mRNA 丰度。此外，Met-Met 增加了空肠和回肠中紧密连接蛋白的表达，减少了与细胞凋亡相关的蛋白表达。这些结果表明，Met-Met 可促进蛋氨酸缺乏的小鼠的肠道功能，优于单独使用蛋氨酸。
