Sörén L, Maller R
Scand J Immunol. 1979;9(1):1-7. doi: 10.1111/j.1365-3083.1979.tb02699.x.
Lymphocytes stimulated with purified protein derivative (PPD) were inhibited in their response to a second stimulation with PPD or phytohaemagglutinin (PHA). The degree of inhibition was related to the PPD concentration during prestimulation, the dose-response curve for inhibition resembling very much that of stimulation. The decreased reactivity was neither due to a toxic effect of PPD nor to altered proliferation kinetics of the prestimulated cells. Lymphocytes preincubated for 6 h or 16 days with PPD were equally refractory, and the non-reactivity persisted even if the cells were incubated without stimulant for 1 week or more. The prestimulated cells were able to suppress the PHA stimulation of fresh lymphocytes. These results indicate that the decreased reactivity of the prestimulated lymphocytes is due to the action of suppressor lymphocytes, generated during the primary stimulation.
用纯化蛋白衍生物（PPD）刺激的淋巴细胞，对PPD或植物血凝素（PHA）的第二次刺激反应受到抑制。抑制程度与预刺激期间的PPD浓度有关，抑制的剂量反应曲线与刺激的剂量反应曲线非常相似。反应性降低既不是由于PPD的毒性作用，也不是由于预刺激细胞增殖动力学的改变。用PPD预孵育6小时或16天的淋巴细胞同样具有抗性，即使细胞在无刺激物的情况下孵育1周或更长时间，无反应性仍然存在。预刺激的细胞能够抑制新鲜淋巴细胞的PHA刺激。这些结果表明，预刺激淋巴细胞反应性降低是由于初次刺激期间产生的抑制性淋巴细胞的作用。
