抗-DR分型试剂的筛查程序。

A screening program for anti-DR typing reagents.

作者信息

Vassalli P, Jeannet M, de Moerloose P, Chardonnens X

出版信息

Tissue Antigens. 1979 Jan;13(1):77-80. doi: 10.1111/j.1399-0039.1979.tb01142.x.

DOI:10.1111/j.1399-0039.1979.tb01142.x

Abstract

A total of 694 sera have been tested in a screening program aimed at identifying monospecific reagents for HLA--DR typing. All sera were first tested on a panel of enriched B and T cells without absorption on platelets. Only sera reacting more frequently on B than on T lymphocytes were absorbed on platelets and retested on the panel. This procedure saved a considerable amount of platelets and proved to be reasonably efficient. One-hundred-and-fifty sera were found to contain an anti-B cell antibody. Significantly less frequent B cell reactivity was found when HLA--A, --B, --C antibodies could not be detected. Twenty-five sera were demonstrated to be specific for well-defined DR antigens.

摘要

在一项旨在鉴定用于HLA - DR分型的单特异性试剂的筛查项目中，共检测了694份血清。所有血清首先在一组富集的B细胞和T细胞上进行检测，且未在血小板上进行吸收处理。只有那些在B细胞上反应比在T淋巴细胞上更频繁的血清才在血小板上进行吸收处理，然后在该细胞组上重新检测。这一程序节省了大量血小板，且证明相当有效。发现有150份血清含有抗B细胞抗体。当检测不到HLA - A、 - B、 - C抗体时，B细胞反应明显较少见。有25份血清被证明对明确的DR抗原具有特异性。