Seignalet J
Rev Fr Transfus Immunohematol. 1980 Sep;23(4):411-25. doi: 10.1016/s0338-4535(80)80167-x.
Screening of anti HLA-DR sera was obtained without absorptions of sera on platelets. In the first place, all our sera were tested by a lymphocytotoxicity technic on 20 T lymphocytes varieties, containing all the known HLA-A, B, C antigens. Anti HLA-A, B, C antibodies are isolated and excluded. Other sera are tested on 20 B lymphocytes varieties and positive sera are harvested, which often contains anti HLA-DR antibodies. HLA-DR typing were performed on 100 healthy not related individuals. The sera were, some from different laboratories (France-Transplant, Lyon), others from Montpellier (37 local sera). A lymphocytes were isolated by adhesivity on nylon wool column. A one stage lymphocytotoxicity technic was used for typing. Anti HLA-DR sera often are polyspecific and often contain antibodies with unknown specificity. The frequency of seven classical antigens, from DRw1 to DRw7 was established in the Languedoc populations. The antigens distribution is close to that observed in Europe, except for DRw1 which is more frequent. Several cross reactions between HLA-DR antigens were noticed.
在未用血小板吸附血清的情况下获得了抗HLA - DR血清。首先，我们所有的血清都通过淋巴细胞毒性技术在20种T淋巴细胞品种上进行检测，这些品种包含所有已知的HLA - A、B、C抗原。分离并排除抗HLA - A、B、C抗体。其他血清在20种B淋巴细胞品种上进行检测，收获阳性血清，这些血清通常含有抗HLA - DR抗体。对100名健康无关个体进行了HLA - DR分型。血清中，一些来自不同实验室（法国移植中心，里昂），另一些来自蒙彼利埃（37份当地血清）。通过尼龙毛柱上的黏附作用分离淋巴细胞。采用一步法淋巴细胞毒性技术进行分型。抗HLA - DR血清通常具有多特异性，且常常含有特异性未知的抗体。在朗格多克人群中确定了从DRw1到DRw7这七种经典抗原的频率。除了DRw1频率更高外，抗原分布与在欧洲观察到的情况相近。还注意到了HLA - DR抗原之间的几种交叉反应。
