School of Pharmacy, Lanzhou University, LanZhou, 730000, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
Molecules. 2019 May 18;24(10):1923. doi: 10.3390/molecules24101923.
Licorice, the root and rhizome of Glycyrrhiza uralansis Fisch, is one of the most frequently used Traditional Chinese Medicines in rigorous clinical trials to remove toxins and sputum, and to relieve coughing. However, the aerial parts are not used so widely at present. It has been reported that the aerial parts have many bioactivities such as anti-microbial and anti-HIV activities. In this study, we aimed to discover the bioactive compounds from the leaves of G. uralensis. Four new compounds, licostilbene A-B (1-2) and licofuranol A-B (3-4), together with eight known flavonoids (5-12), were isolated and identified from the leaves of G. uralensis. Their structures were elucidated mainly by the interpretation of high-resolution electrospray mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR) spectroscopic data. Compared with quercetin, which showed a 50% inhibitory concentration (IC) value of 4.08 μg/mL, compounds 1-9 showed significant anti-inflammatory activities by inhibiting lipopolysaccharide (LPS)-induced nitric oxide (NO) production with IC values of 2.60, 2.15, 3.21, 3.25, 2.00, 3.45, 2.53, 3.13 and 3.17 μg/mL, respectively. The discovery of these active compounds is important for the prevention and treatment of inflammation.
甘草，是豆科甘草属植物乌拉尔甘草的根及根茎，是目前在严格临床试验中用于解毒祛痰、止咳最常用的中药之一。然而，其地上部分目前应用并不广泛。据报道，甘草地上部分具有多种生物活性，如抗微生物和抗 HIV 活性。在这项研究中，我们旨在从甘草属植物的叶子中发现具有生物活性的化合物。从甘草属植物的叶子中分离并鉴定了四个新化合物，即 licostilbene A-B（1-2）和 licofuranol A-B（3-4），以及八个已知的类黄酮（5-12）。它们的结构主要通过高分辨率电喷雾质谱（HR-ESI-MS）和核磁共振（NMR）谱数据的解析来阐明。与槲皮素相比，其 50%抑制浓度（IC）值为 4.08μg/mL，化合物 1-9 通过抑制脂多糖（LPS）诱导的一氧化氮（NO）产生显示出显著的抗炎活性，IC 值分别为 2.60、2.15、3.21、3.25、2.00、3.45、2.53、3.13 和 3.17μg/mL。这些活性化合物的发现对于炎症的预防和治疗具有重要意义。
