Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Mexico.
Faculty of Medicine, Department of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada.
Biomed Res Int. 2019 Apr 11;2019:3286489. doi: 10.1155/2019/3286489. eCollection 2019.
Cav. (Onagraceae) has been used in Mexican traditional medicine to alleviate stomachache, biliary colic, urine retention, stomach cancer, and skin, dental, buccal, and urinary infections. The objective of this study was to determine the bioactivities of specific parts of the plant to scientifically confirm its traditional use. Aerial parts and flowers were macerated and subsequently extracted with hexane, chloroform, and methanol. This study was focused on the analysis of polar components, and thus the methanolic fractions were selected for further investigations. These fractions were evaluated for their antimicrobial activity using a panel of bacterial Gram-positive and -negative strains, as well as fungal strains, including filamentous fungi and yeasts. In addition, the cytotoxic activity of the extract was assessed by MTT using the human-derived monocytic THP-1 and the normal human fibroblast cell lines. Various fractions showed antimicrobial activity against various pathogens, although the most relevant were against and . No inhibition of yeasts was recorded. Only four fractions showed cytotoxic effects when the human-derived THP-1 and fibroblast cells were assessed. The four flavonoids isolated from the extract were luteolin, luteolin-6-C-hexoside, luteolin-8-C-hexoside, and hyperoside. The biological activities presented in this study validate some traditional uses of the plant.
Cav.（Onagraceae）在墨西哥传统医学中被用于缓解胃痛、胆绞痛、尿潴留、胃癌以及皮肤、牙科、口腔和尿路感染。本研究的目的是确定植物特定部位的生物活性，以科学证实其传统用途。地上部分和花经过浸渍，然后用己烷、氯仿和甲醇提取。本研究专注于分析极性成分，因此选择甲醇馏分进行进一步研究。这些馏分的抗菌活性使用一组革兰氏阳性和阴性细菌菌株以及真菌菌株，包括丝状真菌和酵母进行评估。此外，使用 MTT 评估提取物对人源性单核细胞 THP-1 和正常人类成纤维细胞系的细胞毒性。各种馏分对各种病原体均显示出抗菌活性，尽管最相关的是针对 和 。未记录到对酵母的抑制作用。只有四种馏分在评估人源性 THP-1 和成纤维细胞时表现出细胞毒性作用。从提取物中分离出的四种类黄酮为木樨草素、木樨草素-6-C-己糖苷、木樨草素-8-C-己糖苷和金丝桃苷。本研究中呈现的生物活性验证了该植物的一些传统用途。
