Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
Biol Trace Elem Res. 2020 Feb;193(2):456-465. doi: 10.1007/s12011-019-01729-z. Epub 2019 May 20.
Healing of injuries caused by exposure to heat has been discussed in many studies, although a few drugs have been shown to produce satisfactory results. In this study, 100 healthy mice randomly allocated into four categories (each = 25 mice) were analyzed. A deep second-degree burn on the back of each mouse was created. The burns were dressed daily with either AgNPs or silver sulfadiazine over 28 days of treatment. Safety evaluation of the AgNP treatment was performed by measuring the deposition rate of silver in the liver, brain, and kidney of treated mice. In the murine burn model, the speed of wound healing and the antibacterial effect of AgNPs were better than those in the silver sulfadiazine group. Burn wounds treated with SSD appeared to display a greater degree of inflammation as notable by the three clinical signs of the inflammatory process such as redness and swelling which appeared to be less after wounds treated with AgNPs. Also, AgNP treatment modified leukocytic infiltration and reduced collagen degeneration in treated mice and enhanced healing processes that were confirmed by morphological and histological investigations. Beside the potential significant effects of AgNPs on reduction of some microorganism counts that routinely isolated from burn wounds included aerobic organisms as Staphylococcus aureus and Escherichia coli when compared to both SSD and control groups. The deposition kinetics of AgNPs revealed lower distribution in the liver, brain, and kidney than that in silver sulfadiazine-treated mice with respect to both SSD and control groups.
尽管已有少数药物被证明能产生令人满意的效果，但许多研究都讨论了暴露于热引起的损伤的愈合。在这项研究中，分析了 100 只随机分为四组（每组 = 25 只）的健康小鼠。在每只小鼠的背部造成深二度烧伤。在 28 天的治疗过程中，每天用载银纳米颗粒或磺胺嘧啶银处理这些烧伤。通过测量治疗小鼠的肝脏、大脑和肾脏中银的沉积率，对载银纳米颗粒治疗的安全性进行了评估。在小鼠烧伤模型中，载银纳米颗粒在促进伤口愈合和抗菌方面的效果优于磺胺嘧啶银组。用 SSD 处理的烧伤伤口似乎表现出更大程度的炎症，三个炎症过程的临床迹象（如红肿）表明，用载银纳米颗粒处理后的炎症程度较低。此外，载银纳米颗粒治疗还改变了治疗小鼠的白细胞浸润，减少了胶原变性，并通过形态学和组织学研究证实了愈合过程的增强。除了载银纳米颗粒对减少一些常规从烧伤伤口中分离的微生物数量的潜在显著影响外，与 SSD 和对照组相比，载银纳米颗粒还降低了需氧菌，如金黄色葡萄球菌和大肠杆菌的数量。与 SSD 和对照组相比，载银纳米颗粒在肝脏、大脑和肾脏中的分布均低于磺胺嘧啶银处理的小鼠。
