通过双向凝胶电泳分析急性淋巴细胞白血病淋巴母细胞中的细胞蛋白质。

Analysis of cell proteins in lymphoblasts of acute lymphocytic leukemia by two-dimensional gel electrophoresis.

作者信息

Tsuchiya H, Migita M, Nunoi H, Adachi N, Matsuda I, Takeda T

出版信息

Acta Haematol. 1987;77(2):65-71. doi: 10.1159/000205956.

DOI:10.1159/000205956

Abstract

The two-dimensional gel electrophoresis (2-DE) method developed by O'Farrell was used to analyze the differences of major proteins between lymphoblasts from children with acute lymphocytic leukemia (ALL) at diagnosis (lymphoblasts, n = 6), normal lymphocytes (n = 6) and lymphocytes from children with ALL in complete remission (lymphocytes in CR, n = 12). Among all the spots, those which were deeply stained and large were termed 'major spots'. Their number was 118. Two spots (No. B1 and B2) were characteristic of lymphoblasts, three spots (No. N1-N3) were characteristic of normal lymphocytes. In lymphocytes in CR, the latter spots were not always detected, and furthermore, the former spots were detected in several gels. These findings may relate to the insufficient recovery of functions of lymphocytes in CR. Several spots (No. 6, 55 and 57) in lymphoblasts were remarkably larger than those in normal lymphocytes. Spot No. 67, an actin spot, in lymphoblasts was smaller than that in normal lymphocytes. Spots No. 6, 55 and 57 in lymphocytes in CR were relatively small in size.

摘要

采用奥法雷尔开发的双向凝胶电泳（2-DE）方法，分析急性淋巴细胞白血病（ALL）患儿诊断时的淋巴母细胞（淋巴母细胞，n = 6）、正常淋巴细胞（n = 6）和完全缓解期ALL患儿的淋巴细胞（CR期淋巴细胞，n = 12）之间主要蛋白质的差异。在所有斑点中，染色深且大的斑点被称为“主要斑点”。其数量为118个。两个斑点（编号B1和B2）是淋巴母细胞的特征性斑点，三个斑点（编号N1 - N3）是正常淋巴细胞的特征性斑点。在CR期淋巴细胞中，后一组斑点并非总能检测到，此外，前一组斑点在几块凝胶中也能检测到。这些发现可能与CR期淋巴细胞功能恢复不足有关。淋巴母细胞中的几个斑点（编号6、55和57）明显大于正常淋巴细胞中的斑点。淋巴母细胞中的编号67斑点（肌动蛋白斑点）小于正常淋巴细胞中的斑点。CR期淋巴细胞中的编号6、55和57斑点尺寸相对较小。