Ozkan Ufuk, Akal Ali, Ozkan Kudret, Yilmaz Omer Faruk, Adıbelli Fatih Mehmet
Department of Ophthalmology, Sanliurfa Mehmet Akif Inan Training and Research Hospital, Sanliurfa, Turkey.
Department of Ophthalmology, Harran University School of Medicine, Sanliurfa, Turkey.
Arq Bras Oftalmol. 2019 May 20;82(4):322-328. doi: 10.5935/0004-2749.20190067.
We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue.
In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum.
Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p<0.001) and between the cefuroxime and balanced salt solution groups (p<0.001). Furthermore, significant differences were also observed in oxidative stress index levels between the cefuroxime and balanced salt solution groups (p=0.001) and between the cefuroxime and sham groups (p=0.026). According to the immunohistochemical staining results, a significant association with caspase-3 activity existed between the cefuroxime and balanced salt solution groups (p=0.007), while no significant difference was found with caspase-8 activity (p=0.541). Caspase-3 activity exhibited a significant relationship between the sham and balanced salt solution groups (p=0.018), but no relationship was found with caspase-8 activity (p=0.623).
Immunohistochemical examination revealed that intracameral cefuroxime increased apoptosis when compared to the sham and balanced salt solution groups. Moreover, intracameral cefuroxime increased oxidative stress in the cornea and simultaneously induced apoptosis.
我们研究了前房内注射头孢呋辛对大鼠角膜组织氧化应激和内皮细胞凋亡的影响。
总共30只大鼠被分为3组，每组10只（前房内注射0.1mg/0.01mL头孢呋辛（头孢呋辛组）；前房内注射0.01mL平衡盐溶液（对照组）；或不进行前房内注射（假手术组）。使用半胱天冬酶-3和半胱天冬酶-8通过免疫组织化学分析评估角膜内皮细胞凋亡。检测角膜内皮组织和血清中的总氧化剂状态、总抗氧化剂状态、氧化应激指数以及对氧磷酶和芳基酯酶水平。
假手术组和头孢呋辛组之间血清中的对氧磷酶水平存在显著差异（p = 0.027）。头孢呋辛组和平衡盐溶液组之间的总氧化剂状态水平也观察到显著差异（p = 0.023）。此外，头孢呋辛组和假手术组之间角膜组织中的总抗氧化剂状态水平存在显著差异（p < 0.001），头孢呋辛组和平衡盐溶液组之间也存在显著差异（p < 0.001）。此外，头孢呋辛组和平衡盐溶液组之间以及头孢呋辛组和假手术组之间的氧化应激指数水平也观察到显著差异（p = 0.001和p = 0.026）。根据免疫组织化学染色结果，头孢呋辛组和平衡盐溶液组之间半胱天冬酶-3活性存在显著相关性（p = 0.007），而半胱天冬酶-8活性未发现显著差异（p = 0.541）。假手术组和平衡盐溶液组之间半胱天冬酶-3活性呈现显著关系（p = 0.018），但与半胱天冬酶-8活性未发现关系（p = 0.623）。
免疫组织化学检查显示，与假手术组和平衡盐溶液组相比，前房内注射头孢呋辛会增加细胞凋亡。此外，前房内注射头孢呋辛会增加角膜中的氧化应激并同时诱导细胞凋亡。
