Akal Ali, Ulas Turgay, Goncu Tugba, Guldur Muhammet Emin, Kocarslan Sezen, Taskin Abdullah, Savik Emin, Ozkan Ufuk, Karakas Emel Yigit, Koksal Mete, Aksoy Nurten
Department of Ophthalmology .
Cutan Ocul Toxicol. 2015;34(2):139-43. doi: 10.3109/15569527.2014.918138. Epub 2014 Jun 25.
In this experimental study, we investigated the possible effects of intracameral moxifloxacin on oxidative stress parameters and endothelial cell morphology in corneal tissue.
In total, 30 rats were randomly assigned to three groups of 10 rats: the sham group (Group 1, n = 10); the control group (Group 2), where balanced salt solution (BSS) was administered at a dose of 0.01 cc (n = 10); and the treatment group (Group 3), where moxifloxacin was administered at a dose of 0.05 mg/0.01 cc (n = 10). Total antioxidant status (TAS) and total oxidant status (TOS) in corneal tissue and blood samples were measured and the oxidative stress index (OSI) was calculated. Also, corneal tissue histopathology was evaluated with caspase-3 and caspase-8 staining. Apoptotic activity was also evaluated.
In blood samples, TAS, TOS, and OSI levels were not statistically significantly different (all p > 0.05). Compared with the sham and control groups, TOS and OSI levels in cornea tissue were significantly different in the moxifloxacin group (all p < 0.05). However, compared with the control group, no statistically significant difference was found in the sham group (all p > 0.05). Compared with the sham and control groups, apoptotic activity was higher in the moxifloxacin group, in both immunohistochemical staining for caspase-3 and caspase-8.
Intracameral moxifloxacin injection seems to be safe systemically, but it may have toxic effects on corneal tissues, as suggested by oxidative stress parameters and a histopathological evaluation.
在本实验研究中，我们探究了前房内注射莫西沙星对角膜组织氧化应激参数和内皮细胞形态的可能影响。
总共30只大鼠被随机分为三组，每组10只：假手术组（第1组，n = 10）；对照组（第2组），以0.01 cc的剂量给予平衡盐溶液（BSS）（n = 10）；治疗组（第3组），以0.05 mg/0.01 cc的剂量给予莫西沙星（n = 10）。测量角膜组织和血液样本中的总抗氧化状态（TAS）和总氧化状态（TOS），并计算氧化应激指数（OSI）。此外，用半胱天冬酶-3和半胱天冬酶-8染色评估角膜组织的组织病理学。还评估了细胞凋亡活性。
在血液样本中，TAS、TOS和OSI水平无统计学显著差异（所有p>0.05）。与假手术组和对照组相比，莫西沙星组角膜组织中的TOS和OSI水平有显著差异（所有p<0.05）。然而，与对照组相比，假手术组未发现统计学显著差异（所有p>0.05）。与假手术组和对照组相比，在半胱天冬酶-3和半胱天冬酶-8的免疫组织化学染色中，莫西沙星组的细胞凋亡活性更高。
前房内注射莫西沙星似乎全身是安全的，但如氧化应激参数和组织病理学评估所示，它可能对角膜组织有毒性作用。
