Margolis J
Dev Biol Stand. 1987;67:163-7.
Factor VIII-deficient plasma (natural or artificial) mixed with kaolin and phospholipid can be lyophilized to provide ready-to-use substrate which is stable for months at 4 degrees C and usable after many weeks at room temperature. Factor VIII assays are much simplified and more reproducible using this reagent and can be quantified with the aid of a programmable calculator according to the equation (formula; see text) as % of standard and X, S and B are clotting times of test, standard and blank samples respectively. The slope of the log/log function (k) is approximately--6.5.
与高岭土和磷脂混合的 VIII 因子缺乏血浆（天然或人工）可冻干制成即用型底物，该底物在 4℃下可稳定保存数月，在室温下放置数周后仍可使用。使用该试剂，VIII 因子检测大大简化且更具可重复性，可借助可编程计算器根据公式（见正文）将其定量为标准品的百分比，其中 X、S 和 B 分别为测试样品、标准品和空白样品的凝血时间。对数/对数函数的斜率（k）约为 -6.5。
