Barrowcliffe T W, Gray E
Thromb Haemost. 1981 Oct;46(3):629-33.
Some general properties of phospholipid reagents have been studied, particularly in relation to their use in detection and measurement of Factor VIII deficiency. There was wide variation among reagents in their total phospholipid concentration, and in the degree of contamination with tissue factor and oxidation products. Although clotting times of normal and haemophilic plasma were affected by change of phospholipid concentration, presence of oxidation products, and order of addition of reagents, these factors did not appear to influence the efficiency of detection of the Factor VIII defect. The method of activation had a definite influence on sensitivity to Factor VIII deficiency, both the type of activator and the time of activation being important. In Factor VIII one-stage assays, use of different phospholipids with the same substrate plasma gave significant differences in precision, but the estimates of potency of a Factor VIII concentrate against a plasma standard were not significantly different.
已经研究了磷脂试剂的一些一般特性，特别是它们在检测和测量因子VIII缺乏症中的应用。试剂之间在总磷脂浓度、组织因子和氧化产物的污染程度方面存在很大差异。尽管正常血浆和血友病血浆的凝血时间受磷脂浓度变化、氧化产物的存在以及试剂添加顺序的影响，但这些因素似乎并未影响因子VIII缺陷的检测效率。激活方法对因子VIII缺乏症的敏感性有明确影响，激活剂类型和激活时间都很重要。在因子VIII一步法测定中，使用相同底物血浆搭配不同磷脂，精密度有显著差异，但因子VIII浓缩物相对于血浆标准品的效价估计值并无显著差异。
