Saito M, Toyoshima S, Osawa T
Biochem J. 1978 Dec 1;175(3):823-31. doi: 10.1042/bj1750823.
A sialoglycoprotein with an approx. mol.wt. of 95000 was isolated from human lymphoblastoid cells of a MOLT-4B cell line, which was of human T-lymphocyte origin, by ion-exchange chromatography, affinity chromatography on a column of wheat-germ agglutinin-Sepharose and preparative slab-gel electrophoresis. The localization of this glycoprotein on the cell surface was indicated by surface labelling by the periodate/NaB3H4 and lactoperoxidase-catalysed iodination methods. Carbohydrate analyses of this glycoprotein revealed that its total carbohydrate content is 28% (w/w), and it contains fucose, galactose, mannose, N-acetylglucosamine, N-acetylgalactosamine and sialic acid in molar proportions 1.0:4.0:3.7:3.5:1.2:2.5, suggesting that it has two types of sugar chain, i.e. sugar chains like those of serum glycoproteins and sugar chains of the type found in mucins. Actually, alkaline borohydride treatment of this glycoprotein yielded tri- and tetra-saccharide, the latter containing 1 molecule of fucose in addition to each molecule of galactose, N-acetylgalactosamine and sialic acid. This glycoprotein bound to Ricinus communis agglutinin and concanavalin A as well as to wheat-germ agglutinin.
从人T淋巴细胞来源的MOLT - 4B细胞系的人淋巴母细胞中，通过离子交换色谱法、麦胚凝集素 - 琼脂糖柱亲和色谱法和制备性平板凝胶电泳，分离出一种分子量约为95000的唾液糖蛋白。通过高碘酸盐/NaB3H4表面标记法和乳过氧化物酶催化碘化法进行表面标记，表明该糖蛋白定位于细胞表面。对该糖蛋白的碳水化合物分析显示，其总碳水化合物含量为28%（w/w），并且含有岩藻糖、半乳糖、甘露糖、N - 乙酰葡糖胺、N - 乙酰半乳糖胺和唾液酸，摩尔比例为1.0:4.0:3.7:3.5:1.2:2.5，这表明它具有两种类型的糖链，即类似于血清糖蛋白的糖链和粘蛋白中发现的那种类型的糖链。实际上，用碱性硼氢化物处理该糖蛋白可产生三糖和四糖，后者除了每个半乳糖、N - 乙酰半乳糖胺和唾液酸分子外还含有1分子岩藻糖。该糖蛋白与蓖麻凝集素、伴刀豆球蛋白A以及麦胚凝集素结合。
