Judd W, Poodry C A, Strominger J L
J Exp Med. 1980 Nov 1;152(5):1430-5. doi: 10.1084/jem.152.5.1430.
Radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to study the distribution on human lymphoid cells of a previously undescribed surface antigen recognized by several heteroantisera. A glycoprotein with a 90,000 mol wt (under reducing conditions) was detected on all cell lines tested including T, B, null, and myeloid cell lines, although the amount of antigen present varied considerably. The antigen was absent from normal peripheral blood lymphocytes (PBL), B and T cells, monocytes, granulocytes, thymocytes, and erythrocytes. After stimulation with lectins or allogeneic B cells, the antigen was induced on PBL T cells. A limited number of leukemic T cells tested all expressed the antigen, as did melanoma cell line and human embryonic lung fibroblasts. Hence, the antigen was present only on dividing lymphoid cells and absent from nondividing cells, but was also present on the two examples of dividing non-lymphoid cells tested. Under nonreducing conditions, the 90,000-mol wt band normally present disappeared to be replaced by another at approximately 200,000 mol wt. The glycoprotein bound to lectins from lentil and ricin, but not to wheat germ agglutinin. It could be readily labeled metabolically by [35S]methionine or by surface iodination, and appeared to be a major membrane protein on some cell lines.
采用放射免疫沉淀法，随后进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，以研究几种异种抗血清识别的一种先前未描述的表面抗原在人淋巴细胞上的分布情况。在所有测试的细胞系中均检测到一种还原条件下分子量为90,000的糖蛋白，这些细胞系包括T细胞系、B细胞系、裸细胞系和髓细胞系，尽管抗原的含量差异很大。正常外周血淋巴细胞（PBL）、B细胞、T细胞、单核细胞、粒细胞、胸腺细胞和红细胞中均不存在该抗原。用凝集素或同种异体B细胞刺激后，PBL T细胞上可诱导出该抗原。所检测的有限数量的白血病T细胞均表达该抗原，黑色素瘤细胞系和人胚胎肺成纤维细胞也表达该抗原。因此，该抗原仅存在于正在分裂的淋巴细胞上，非分裂细胞中不存在，但在测试的两种正在分裂的非淋巴细胞中也存在。在非还原条件下，通常存在的90,000分子量条带消失，取而代之的是另一条约200,000分子量的条带。该糖蛋白与扁豆和蓖麻的凝集素结合，但不与麦胚凝集素结合。它可以很容易地通过[35S]甲硫氨酸进行代谢标记或通过表面碘化进行标记，并且在某些细胞系中似乎是一种主要的膜蛋白。
