Le Gros J E, Jenkins D R, Prestidge R L, Watson J D
Immunol Cell Biol. 1987 Feb;65 ( Pt 1):57-69. doi: 10.1038/icb.1987.7.
Two cloned murine cell lines, FD.C/1 and 32Dcl-23 exhibit switching of lymphokine-dependent growth states. The bone marrow-derived FD.C/1 and 32Dcl-23 cell lines are normally grown in culture medium supplemented with interleukin 3 (IL3). The replacement of IL3 with interleukin 2 (IL2) in the medium results in an increase in IL2 receptor expression in FD.C/1 and 32Dcl-23 cells and the switching of cells to an IL2-dependent growth state. We have compared patterns of protein and phosphoprotein synthesis, as well as the expression of the c-abl, c-ras, c-myb, and c-fos oncogenes in these cell lines maintained in IL3- and IL2-dependent growth states. The synthesis of a series of proteins and phosphoproteins are identified with each of the lymphokine-dependent growth states. All of the oncogenes examined are expressed in both IL2- and IL3-dependent cells and are not altered by phenotypic changes in lymphokine growth dependence. The relationship of oncogene expression to intracellular pathways regulated by lymphokine-receptor interactions is considered.
两种克隆的小鼠细胞系，FD.C/1和32Dcl-23表现出淋巴因子依赖性生长状态的转换。源自骨髓的FD.C/1和32Dcl-23细胞系通常在添加白细胞介素3（IL3）的培养基中生长。培养基中用白细胞介素2（IL2）替代IL3会导致FD.C/1和32Dcl-23细胞中IL2受体表达增加，并且细胞转换为IL2依赖性生长状态。我们比较了维持在IL3依赖性和IL2依赖性生长状态下的这些细胞系中蛋白质和磷蛋白的合成模式，以及c-abl、c-ras、c-myb和c-fos癌基因的表达。一系列蛋白质和磷蛋白的合成与每种淋巴因子依赖性生长状态相关。所检测的所有癌基因在IL2依赖性和IL3依赖性细胞中均有表达，并且不会因淋巴因子生长依赖性的表型变化而改变。我们还考虑了癌基因表达与由淋巴因子-受体相互作用调节的细胞内途径之间的关系。
