Garland J M, Kinnaird A
Lymphokine Res. 1986;5 Suppl 1:S145-50.
We have investigated the promotion of two IL3 dependent cell lines by IL3 and a rat factor derived from stimulated spleen cells. Both lines respond to purified IL3, but do not respond to IL2, GM-CSF contained in lung conditioned medium nor to Human IL2. Both lines respond to a factor in rat lymphocyte conditioned media as determined by a standard assay used to detect IL3. However, 123 cell line cells continue to proliferate whereas AC2 cells decline, due to different sensitivities of the lines to rat factor. 123 cells can be re-cloned in the rat factor and exhibit enhanced responses to IL3. The changing kinetics of cell promotion during the first 48 hours question the validity of the standard assay which is widely used, and the specificity of the mechanism by which the cells are promoted.
我们研究了白细胞介素3（IL3）以及一种源自受刺激脾细胞的大鼠因子对两种依赖IL3的细胞系的促生长作用。这两种细胞系均对纯化的IL3有反应，但对IL2、肺条件培养基中所含的粒细胞-巨噬细胞集落刺激因子（GM-CSF）以及人IL2均无反应。通过用于检测IL3的标准测定法确定，这两种细胞系均对大鼠淋巴细胞条件培养基中的一种因子有反应。然而，由于这两种细胞系对大鼠因子的敏感性不同，123细胞系的细胞持续增殖，而AC2细胞则数量减少。123细胞可以在大鼠因子中进行再克隆，并对IL3表现出增强的反应。在最初48小时内细胞促生长的变化动力学对广泛使用的标准测定法的有效性以及细胞促生长机制的特异性提出了质疑。
