Departamento Patología Animal I (Sanidad Animal). Facultad de Veterinaria, Universidad Complutense, 28040, Madrid, Spain.
Mycotoxin Res. 1994 Mar;10(1):15-20. doi: 10.1007/BF03192247.
In the present work we studied the ability of phosphate buffer to solubize sterigmatocystin (ST) at different pH values. We observed a higher solubility of ST at acid pH values, specially in pH 4.5. The ST adsorbed to glass was maximum at alkaline pH values.Also we studied the stability of different concentrations of ST in phosphate buffer at different pH values. After 24 h, ST can be considered stable at alkaline, neutral and 3.5 pH values. At pH 4.5, 5.5 and 6.5, ST recovered after 24 h was lower, specially at pH 4.5, when only 44% was recovered. At day 7, ST was stable only at pH 7.5 (91%). The lowest recovery was at pH 4.5 (25%).
在本工作中,我们研究了磷酸盐缓冲液在不同 pH 值下溶解桔青霉素(ST)的能力。我们观察到 ST 在酸性 pH 值下具有更高的溶解度,特别是在 pH 值为 4.5 时。ST 吸附在玻璃上的量在碱性 pH 值下达到最大值。此外,我们还研究了不同浓度的 ST 在不同 pH 值的磷酸盐缓冲液中的稳定性。24 小时后,ST 在碱性、中性和 3.5 pH 值下可以被认为是稳定的。在 pH 值为 4.5、5.5 和 6.5 时,24 小时后回收的 ST 较低,特别是在 pH 值为 4.5 时,仅回收了 44%。在第 7 天,ST 仅在 pH 值为 7.5(91%)时稳定。回收率最低的是在 pH 值为 4.5(25%)时。