Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.
Division of Bio-Medical Science and Technology (Biological Chemistry), Korea University of Science and Technology (UST), Daejeon 34113, Korea.
Molecules. 2019 May 22;24(10):1977. doi: 10.3390/molecules24101977.
Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods. The use of CE-LIF in immunoassays and enzyme assays has gained a reputation in recent years for its high detection sensitivity, short analysis time, and accurate quantification. Immunoassays are bioassay platforms that rely on binding reactions between an antigen (analyte) and a specific antibody. Enzyme assays measure enzymatic activity through quantitative analysis of substrates and products by the reaction of enzymes in purified enzyme or cell systems. These two category analyses play an important role in the context of biopharmaceutical analysis, clinical therapy, drug discovery, and diagnosis analysis. This review discusses the expanding portfolio of immune and enzyme assays using CE-LIF and focuses on the advantages and disadvantages of these methods over the ten years of existing technology since 2008.
毛细管电泳激光诱导荧光检测（CE-LIF）是电分离方法中最灵敏的分离工具之一。近年来，CE-LIF 在免疫分析和酶分析中的应用因其高检测灵敏度、短分析时间和准确定量而获得了良好的声誉。免疫分析是一种生物测定平台，依赖于抗原（分析物）与特定抗体之间的结合反应。酶分析通过定量分析酶在纯化酶或细胞系统中的反应中底物和产物来测量酶活性。这两种分析在生物制药分析、临床治疗、药物发现和诊断分析中都发挥着重要的作用。本文综述了使用 CE-LIF 的免疫分析和酶分析的扩展组合，并重点讨论了自 2008 年以来，该技术在过去十年中的优势和劣势。
