Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences,Lhasa, Tibet, 850000,China.
Colleges of Life Science and Technology, Dalian University, Dalian Economic Technological Development Zone,Liaoning, 116622,China.
J Dairy Res. 2019 May;86(2):181-187. doi: 10.1017/S0022029919000268. Epub 2019 May 24.
This research paper addresses the hypothesis that Septin6 is a key regulatory factor influencing amino acid (AA)-mediated cell growth and casein synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were treated with absence of AA (AA-), restricted concentrations of AA (AAr) or normal concentrations of AA (AA+) for 24 h. Cell growth, expression of CSN2 and Septin6 were increased in response to AA supply. Overexpressing or inhibiting Septin6 demonstrated that cell growth, expression of CSN2, mTOR, p-mTOR, S6K1 and p-S6K1 were up-regulated by Septin6. Furthermore, overexpressing or inhibiting mTOR demonstrated that the increase in cell growth and expression of CSN2 in response to Septin6 overexpression were inhibited by mTOR inhibition, and vice versa. Our hypothesis was supported; we were able to show that Septin6 is an important positive factor for cell growth and casein synthesis, it up-regulates AA-mediated cell growth and casein synthesis through activating mTORC1 pathway in DCMECs.
本研究论文提出了一个假设，即 Septin6 是一个关键的调节因子，影响氨基酸（AA）介导的奶牛乳腺上皮细胞（DCMEC）的细胞生长和酪蛋白合成。用缺乏 AA（AA-）、限制浓度的 AA（AAr）或正常浓度的 AA（AA+）处理 DCMECs 24 小时。AA 供应会促进细胞生长和 CSN2 和 Septin6 的表达。过表达或抑制 Septin6 表明，细胞生长、CSN2、mTOR、p-mTOR、S6K1 和 p-S6K1 的表达受 Septin6 上调。此外，过表达或抑制 mTOR 表明，mTOR 抑制抑制了 Septin6 过表达对细胞生长和 CSN2 表达的增加，反之亦然。我们的假设得到了支持；我们能够表明 Septin6 是细胞生长和酪蛋白合成的一个重要正因子，它通过激活 DCMECs 中的 mTORC1 途径来上调 AA 介导的细胞生长和酪蛋白合成。
