Yan Shuguang, Deng Dongyan, Zhang Lichun, Lv Yi
Analytical & Testing Center, Sichuan University, Chengdu, Sichuan, 610064, China.
Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, China.
Talanta. 2019 Aug 15;201:96-103. doi: 10.1016/j.talanta.2019.03.107. Epub 2019 Apr 2.
The versatile fluorescence biosensors were implemented based on multipurpose nano metal organic frameworks (nMOFs) nanocomposites, which were obtained as fluorescence signal probes through encapsulation nMOFs. In this modulation process, the encapsulant bind with nMOFs through electrostatic attraction and hydrogen bond. Meanwhile, fluorescence enhancement of nMOFs were observed, accompanied by an increased in water solubility. The specific combination of target analyte triggers the release of the encapsulant and allows the target analyte to bind encapsulant to form a stable analyte-encapsulant complex. The complex binds tightly and promotes the photoinduced electron transfer from MOFs nanocomposites to the complex, thus resulting in reduce the fluorescence intensity of the MOFs nanocomposites. The results indicated that this novel system enables the specific and versatile detection of target biomolecules such as hemin and folic acid with high sensitivity and selectivity based on the choices of different encapsulant. Under optimized conditions, these biosensors show high sensitivity with a linear range from 0.16 to 12.5 μg/mL for hemin and 0.15-17.5 μmol/L for folic acid and detection limit of 47 ng/mL for hemin and 45 nmol/L folic acid. Moreover, it exhibits good performance of excellent selectivity, high stability, and acceptable fabrication reproducibility. This new multipurpose MOFs nanocomposites open avenues for combining together their properties and functionalities, and displaying novel important applications in fluorescence biosensors.
多功能荧光生物传感器是基于多功能纳米金属有机框架（nMOF）纳米复合材料实现的，这些复合材料通过封装nMOF作为荧光信号探针获得。在这个调制过程中，封装剂通过静电吸引和氢键与nMOF结合。同时，观察到nMOF的荧光增强，伴随着水溶性的增加。目标分析物的特异性结合触发封装剂的释放，并使目标分析物与封装剂结合形成稳定的分析物 - 封装剂复合物。该复合物紧密结合并促进光诱导电子从MOF纳米复合材料转移到复合物，从而导致MOF纳米复合材料的荧光强度降低。结果表明，基于不同封装剂的选择，这种新型系统能够以高灵敏度和选择性对诸如血红素和叶酸等目标生物分子进行特异性和多功能检测。在优化条件下，这些生物传感器对血红素的线性范围为0.16至12.5μg/mL，对叶酸为0.15 - 17.5μmol/L，血红素的检测限为47ng/mL，叶酸的检测限为45nmol/L，表现出高灵敏度。此外，它还具有优异的选择性、高稳定性和可接受的制备重现性。这种新型多功能MOF纳米复合材料为结合它们的性质和功能开辟了途径，并在荧光生物传感器中展示了新的重要应用。
