Reichart B A, Novitzky D, Cooper D K, Cunningham M S, Rose A G
J Heart Transplant. 1987 Jan-Feb;6(1):15-22.
En bloc transplantation of the heart and lungs was performed in 15 chacma baboons; the donor organs were stored between 4 and 6 hours before transplantation. The hearts were perfused in the donor animals with 15 ml/kg Wicomb's cardioplegic solution at 4 degrees C, the lungs with either 20 ml/kg 4 degrees C Collins' solution with an added 2.5% dextrose and 12 mEq magnesium sulfate (Collins' solution, group 1, n = 8), Collins' solution plus superoxide dismutase (40,000 U/L superoxide dismutase, group 2, n = 4), or Collins' solution plus superoxide dismutase plus peroxidase (5000 U/L peroxidase plus mannitol, group 3, n = 3). The pulmonary artery perfusion pressure was not allowed to exceed 50 cm water; the lungs were maintained at 30% to 50% inflation, and external cooling was applied. After explantation the thoracic organs were stored in 0.9% saline solution at 4 degrees C. In groups 1 (Collins' solution) and 2 (Collins' plus superoxide dismutase) all surviving baboons revealed normal blood gas values and normal light and electron microscopic histology at 24 hours. Three animals had further biopsies at intervals between 1 and 9 days, at which time the histology of the lungs proved normal and well preserved. All three baboons in group 3 (Collins' plus superoxide dismutase plus peroxidase) had grossly abnormal blood gas values from the time of operation, and all died within 9 hours; light microscopy of the lungs showed early lung infarctlike lesions and in one case pulmonary edema. These preclinical findings proved that storage of the lungs in Collins' solution with or without superoxide dismutase is possible for up to 5 hours; the addition of peroxidase had a detrimental effect.
对15只东非狒狒进行了心肺整块移植；供体器官在移植前保存4至6小时。在供体动物中，心脏用15 ml/kg的4℃维康心脏停搏液灌注，肺用20 ml/kg的4℃柯林斯溶液灌注，该溶液添加了2.5%的葡萄糖和12 mEq硫酸镁（柯林斯溶液，第1组，n = 8）、柯林斯溶液加超氧化物歧化酶（40,000 U/L超氧化物歧化酶，第2组，n = 4）或柯林斯溶液加超氧化物歧化酶加过氧化物酶（5000 U/L过氧化物酶加甘露醇，第3组，n = 3）。肺动脉灌注压力不允许超过50 cm水柱；肺保持30%至50%的膨胀状态，并进行外部冷却。取出后，胸腔器官保存在4℃的0.9%盐溶液中。在第1组（柯林斯溶液）和第2组（柯林斯溶液加超氧化物歧化酶）中，所有存活的狒狒在24小时时血气值正常，光镜和电镜组织学正常。3只动物在1至9天内进行了进一步活检，此时肺组织学证明正常且保存良好。第3组（柯林斯溶液加超氧化物歧化酶加过氧化物酶）的所有3只狒狒从手术时起血气值就严重异常，均在9小时内死亡；肺的光镜检查显示早期肺梗死样病变，1例出现肺水肿。这些临床前研究结果证明，在含有或不含有超氧化物歧化酶的柯林斯溶液中保存肺长达5小时是可行的；添加过氧化物酶有有害作用。
