Berendsen Johanna T W, Eijkel Jan C T, Wetzels Alex M, Segerink Loes I
1BIOS Lab on a Chip Group, MESA+Institute for Nanotechnology, Technical Medical Centre, University of Twente, Enschede, The Netherlands.
2Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Microsyst Nanoeng. 2019 May 20;5:24. doi: 10.1038/s41378-019-0068-z. eCollection 2019.
Men suffering from azoospermia can father a child, by extracting spermatozoa from a testicular biopsy sample. The main complication in this procedure is the presence of an abundance of erythrocytes. Currently, the isolation of the few spermatozoa from the sample is manually performed due to ineffectiveness of filtering methods, making it time consuming and labor intensive. The spermatozoa are smaller in both width and height than any other cell type found in the sample, with a very small difference compared with the erythrocyte for the smallest, making this not the feature to base the extraction on. However, the length of the spermatozoon is 5× larger than the diameter of an erythrocyte and can be utilized. Here we propose a microfluidic chip, in which the tumbling behavior of spermatozoa in pinched flow fractionation is utilized to separate them from the erythrocytes. We show that we can extract 95% of the spermatozoa from a sample containing 2.5% spermatozoa, while removing around 90% of the erythrocytes. By adjusting the flow rates, we are able to increase the collection efficiency while slightly sacrificing the purity, tuning the solution for the available sample in the clinic.
患有无精子症的男性可以通过从睾丸活检样本中提取精子来生育孩子。该过程的主要并发症是存在大量红细胞。目前，由于过滤方法无效，从样本中分离少量精子是手动进行的，这既耗时又费力。精子在宽度和高度上都比样本中发现的任何其他细胞类型小，与最小的红细胞相比差异非常小，因此这不是基于其进行提取的特征。然而，精子的长度比红细胞的直径大5倍，可以加以利用。在此，我们提出一种微流控芯片，其中利用精子在收缩流分馏中的翻滚行为将它们与红细胞分离。我们表明，我们可以从含有2.5%精子的样本中提取95%的精子，同时去除约90%的红细胞。通过调整流速，我们能够在略微牺牲纯度的情况下提高收集效率，针对临床中可用的样本调整解决方案。
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