使用化学成分明确的培养基生成人诱导多能干细胞来源的星形胶质细胞用于体外疾病建模。
Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.
作者信息
Janssen Katharina, Bahnassawy Lamiaa, Kiefer Claudia, Korffmann Jürgen, Terstappen Georg C, Lakics Viktor, Cik Miroslav, Reinhardt Peter
机构信息
Neuroscience Discovery, AbbVie Deutschland GmbH & Co KG, Ludwigshafen am Rhein, Germany.
BG Neurochirurgie und Neurotraumatologie, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil gGmbH, Bochum, Germany.
出版信息
Methods Mol Biol. 2019;1994:31-39. doi: 10.1007/978-1-4939-9477-9_3.
To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not only is chemically defined, that is, it does not use serum, but also allows for the expansion of astrocyte progenitor cells and mature astrocytes. Large batches of hiPSc-derived astrocytes can be stored and used for defined in vitro disease models.
为了更好地理解和模拟神经疾病，尤其是神经退行性疾病，人类诱导多能干细胞（hiPSC）为神经细胞的生成提供了一个很好的来源。我们提供了一种在体外分化hiPSC来源的星形胶质细胞的方案。该方案不仅是化学成分确定的，即不使用血清，而且还允许星形胶质细胞祖细胞和成熟星形胶质细胞的扩增。大量hiPSC来源的星形胶质细胞可以储存起来，并用于特定的体外疾病模型。
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