Department of Obstetrics, Jining No.1 People's Hospital, Jining, China.
J Cell Physiol. 2019 Dec;234(12):22911-22920. doi: 10.1002/jcp.28853. Epub 2019 May 23.
Tumor suppressor long noncoding RNA maternally expressed gene 3 (lncRNA MEG3) exists in various cancers. Nonetheless, the functions of lncRNA MEG3 in choriocarcinoma (CC) are still not well studied. We explored the effects of lncRNA MEG3 on human CC JEG-3 and BeWo cells. lncRNA MEG3 was overexpressed, and the effects of lncRNA MEG3 on cell viability, proliferation, apoptosis, migration, and invasion were assessed by the cell counting kit-8 assay, western blot analysis, flow cytometry (plus western blot analysis), and transwell assay (plus western blot analysis), respectively. Then, the expression level of miR-211 was detected by real-time quantitative polymerase chain reaction. After that, the effects of dysregulated microRNA-211 (miR-211) with overexpressing lncRNA MEG3 on JEG-3 cells and BeWo cells were testified. Western blot analysis was used to study the involvements of the signaling pathways in the lncRNA MEG3-associated modulation. We found that lncRNA MEG3 upregulation reduced cell viability, inhibited proliferation, migration and invasion, and promoted apoptosis. Expression of miR-211 was upregulated after lncRNA MEG3 overexpression. Effects of lncRNA MEG3 overexpression were augmented by miR-211 overexpression, while they were declined by miR-211 silencing. Phosphorylated levels of PI3K, AKT, and AMP-activated protein kinase (AMPK) were decreased by lncRNA MEG3 overexpression via regulation of miR-211. To sum up, lncRNA MEG3 could repress proliferation, migration and invasion, and promote apoptosis of JEG-3 and BeWo cells through upregulating miR-211. The PI3K/AKT and AMPK pathways were inhibited by lncRNA MEG3 overexpression via regulation of miR-211.
肿瘤抑制基因长链非编码 RNA 母系表达基因 3（lncRNA MEG3）存在于各种癌症中。然而，lncRNA MEG3 在绒毛膜癌（CC）中的功能仍未得到很好的研究。我们探讨了 lncRNA MEG3 对人绒毛膜癌细胞系 JEG-3 和 BeWo 细胞的影响。通过细胞计数试剂盒-8 检测、western blot 分析、流式细胞术（结合 western blot 分析）和 Transwell 检测（结合 western blot 分析）分别检测 lncRNA MEG3 过表达对细胞活力、增殖、凋亡、迁移和侵袭的影响，实时定量聚合酶链反应检测 miR-211 的表达水平。然后，检测上调 lncRNA MEG3 后 miR-211 对 JEG-3 细胞和 BeWo 细胞的影响。western blot 分析用于研究 lncRNA MEG3 相关调节中信号通路的参与情况。结果发现，lncRNA MEG3 上调可降低细胞活力，抑制增殖、迁移和侵袭，促进凋亡。lncRNA MEG3 过表达后 miR-211 的表达上调。lncRNA MEG3 过表达的作用被 miR-211 过表达增强，而被 miR-211 沉默减弱。lncRNA MEG3 通过调节 miR-211 降低了 PI3K、AKT 和 AMP 激活蛋白激酶（AMPK）的磷酸化水平。综上所述，lncRNA MEG3 可通过上调 miR-211 抑制 JEG-3 和 BeWo 细胞的增殖、迁移和侵袭，促进凋亡。lncRNA MEG3 通过调节 miR-211 抑制 PI3K/AKT 和 AMPK 通路。
