Department of Food Science and Human Nutrition, University of Florida, Gainesville, Florida, United States of America.
Citrus Research and Education Center, University of Florida, Lake Alfred, Florida, United States of America.
PLoS One. 2019 May 24;14(5):e0217445. doi: 10.1371/journal.pone.0217445. eCollection 2019.
Fresh produce outbreaks due to Shiga toxin-producing Escherichia coli (STEC) continue to occur in the United States (US). Manure-amended soils can pose a public health risk when used for growing raw agricultural commodities. Knowing the prevalence and concentration of STEC in untreated biological soil amendments of animal origin (BSAAO) is important to help guide the most appropriate pre-harvest interval(s) following application to limit risks from these soil amendments. Bovine manure samples were collected from 12 farms in Florida, including samples from piles, lagoons, barns, and screened solids. Two methods were used to detect stx1/2 and rfbE genes in samples. A prevalence rate of 9% for stx1 and/or stx2 and 19% for rfbE was observed from the 518 bovine manure samples evaluated. A most probable number (MPN) assay was performed on stx+ samples when applicable. The geometric mean for stx+ samples (n = 20) was 3.37 MPN g-1 (0.53 log MPN g-1) with a maximum value of 6,800 MPN g-1 (3.83 log MPN g-1). This research was part of a larger nationwide geographical study on the prevalence and concentration of STEC in bovine manure to help guide regulations on feasible pre-harvest intervals for the application of untreated BSAAO.
在美国，由产志贺毒素大肠杆菌（STEC）引起的新鲜农产品爆发仍时有发生。当用于种植生的农产品时，经粪便改良的土壤可能对公共健康构成威胁。了解未经处理的动物源性生物土壤改良剂（BSAAO）中 STEC 的流行率和浓度对于指导最合适的收获前间隔（在应用后）以限制这些土壤改良剂的风险非常重要。从佛罗里达州的 12 个农场收集了牛粪便样本，包括堆肥、泻湖、谷仓和筛选固体的样本。使用两种方法检测样本中的 stx1/2 和 rfbE 基因。从评估的 518 个牛粪便样本中观察到 stx1 和/或 stx2 的流行率为 9%，rfbE 的流行率为 19%。在适用的情况下，对 stx+样品进行最大可能数（MPN）检测。stx+样品的几何平均值（n = 20）为 3.37 MPN g-1（0.53 log MPN g-1），最大值为 6,800 MPN g-1（3.83 log MPN g-1）。这项研究是全国范围内关于牛粪便中 STEC 流行率和浓度的更大范围地理研究的一部分，旨在帮助指导未经处理的 BSAAO 应用的可行收获前间隔的规定。
Zotero 插件