Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, C.U., Cerro de las campanas S/N, Queretaro, Queretaro 76010, Mexico; Campo experimental Zacatecas (CEZAC-INIFAP), Km 24.5 Carretera Zacatecas-Fresnillo, Calera de VR, Zacatecas 98500, Mexico.
Campo experimental Bajío (CEBAJ-INIFAP), Km 6 Carretera Celaya-San Miguel de Allende, 38010 Celaya, Mexico.
Food Chem. 2019 Oct 1;294:368-377. doi: 10.1016/j.foodchem.2019.05.076. Epub 2019 May 9.
Common bean is rich in phytochemicals like polyphenols, saponins, and steroidal glycosides, but its crop is greatly affected by drought. Thus, it is necessary to identify the irrigation regimes that improves its phytochemical profile without affecting seed yield. The aim of this study was to evaluate the differential effect of severe drought (SD), restricted irrigation (RI), and full irrigation (FI) on the phytochemical fingerprint of bean seeds through liquid chromatography-mass spectrometry and chemometric approach. Partial least square-discriminant analysis (PLS-DA) indicated that seeds from plants grown under RI where differentiated from those grown under FI and SD due to their high content of quercetin 3-O-rhamnoside and luteolin 7-O-glucoside. Regarding seed yield, no significant (p < 0.05) differences were observed between RI and FI, whereas SD decreased (p < 0.05) seed yield as compared to FI (47%). These results suggest that mild hydric stress (RI) can be used to induce phytochemicals without affecting seed yield.
菜豆富含多酚、皂甙和甾体糖苷等植物化学物质，但它的作物受干旱影响很大。因此，有必要确定既能改善其植物化学特征又不影响种子产量的灌溉制度。本研究旨在通过液相色谱-质谱联用和化学计量学方法，评估严重干旱 (SD)、限制灌溉 (RI) 和充分灌溉 (FI) 对菜豆种子植物化学特征的差异影响。偏最小二乘判别分析 (PLS-DA) 表明，由于 RI 处理下的菜豆种子中槲皮素 3-O-鼠李糖苷和木樨草素 7-O-葡萄糖苷含量较高，因此与 FI 和 SD 处理下的种子不同。关于种子产量，RI 和 FI 之间没有显著差异（p < 0.05），而与 FI 相比，SD 处理降低了种子产量（p < 0.05，为 47%）。这些结果表明，轻度水分胁迫（RI）可用于诱导植物化学物质而不影响种子产量。
