姜黄素能否改善二氧化钛纳米颗粒对心脏的影响？一项组织病理学、免疫组织化学和遗传毒性研究。
Could curcumin ameliorate titanium dioxide nanoparticles effect on the heart? A histopathological, immunohistochemical, and genotoxic study.
机构信息
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, 44519, Egypt.
Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
出版信息
Environ Sci Pollut Res Int. 2019 Jul;26(21):21556-21564. doi: 10.1007/s11356-019-05433-2. Epub 2019 May 24.
The evaluation of the toxicological effects of titanium dioxide nanoparticles (TiONPs) is increasingly important due to their growing occupational and industrial use. Curcumin is a yellow curry spice with a long history of use in herbal medicine and has numerous protective potentials such as antioxidant, antimicrobial, anti-inflammatory, and anti-apoptotic effects. Accordingly, we tested the hypothesis that curcumin could ameliorate TiONP-induced cardiotoxic and genotoxic effects in adult male albino rats. For this purpose, 48 adult male albino rats were randomized into five groups; all treatment was by oral gavage once daily for 90 days: group I (8 rats), untreated control; group II (16 rats), subdivided into vehicle control IIa (8 rats) received saline and vehicle control IIb (8 rats) received corn oil; group III (8 rats) orally gavaged with curcumin dissolved in 0.5 ml corn oil at a dose of 200 mg/kg b.w./day; group IV treated with TiONPs at a dose of 1200 mg/kg b.w./day (1/10 LD) suspended in 1 ml of 0.9% saline; group V treated with curcumin + TiONPs (the same previously mentioned doses). Curcumin was orally gavaged for 7 days before TiONPs treatment was initiated, and then they received TiONPs along with curcumin at the same doses for 90 days. TiONPs administration resulted in several myocardial cytomorphic changes as structurally disorganized, degenerated, and apoptotic cardiomyocytes and the newly implemented 3-nitrotyrosine immune expression rendered strong evidence that these effects derived from the cardio myocellular oxidative burden. Furthermore, comet assay results confirmed TiONP-related DNA damage. Remarkably, all these changes are partially mitigated in rats treated with both curcumin and TiONPs. Our results suggest that concurrent curcumin treatment has a beneficial role in ameliorating TiONP-induced cardiotoxicity and this may be mediated by its antioxidative property.
由于二氧化钛纳米粒子（TiONPs）的职业和工业用途不断增加，因此对其毒理学效应的评估变得越来越重要。姜黄是一种黄色咖喱香料，在草药医学中已有悠久的使用历史，具有多种保护潜力，如抗氧化、抗菌、抗炎和抗细胞凋亡作用。因此，我们测试了姜黄是否可以改善成年雄性白化大鼠的 TiONP 诱导的心脏毒性和遗传毒性的假说。为此，将 48 只成年雄性白化大鼠随机分为五组；所有治疗均通过口服灌胃，每天一次，持续 90 天：I 组（8 只大鼠），未处理的对照组；II 组（16 只大鼠），细分为 vehicle control IIa（8 只大鼠，给予生理盐水）和 vehicle control IIb（8 只大鼠，给予玉米油）；III 组（8 只大鼠）灌胃溶解于 0.5ml 玉米油中的姜黄，剂量为 200mg/kg b.w./天；IV 组给予 TiONPs，剂量为 1200mg/kg b.w./天（1/10LD），悬浮于 1ml 0.9%生理盐水中；V 组给予姜黄+TiONPs（先前提到的相同剂量）。在开始给予 TiONPs 之前，姜黄通过口服灌胃 7 天，然后在同一剂量下给予 TiONPs 和姜黄 90 天。TiONPs 的给药导致心肌细胞形态发生多种变化，表现为结构紊乱、变性和凋亡的心肌细胞，新实施的 3-硝基酪氨酸免疫表达提供了有力的证据表明这些效应源于心肌细胞的氧化应激。此外，彗星试验结果证实了 TiONP 相关的 DNA 损伤。值得注意的是，用姜黄和 TiONPs 治疗的大鼠的这些变化都得到了部分缓解。我们的结果表明，同时给予姜黄治疗可部分减轻 TiONP 诱导的心脏毒性，这可能与其抗氧化特性有关。
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