Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province 130041, China; Department of Obstetrics and Gynecology, Longnan Hospital of Daqing, Daqing, Heilongjiang Province 163458, China.
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province 130041, China.
Exp Mol Pathol. 2019 Oct;110:104263. doi: 10.1016/j.yexmp.2019.104263. Epub 2019 May 23.
We aimed to investigate if the let-7 s expression level in the serum of peripheral blood from pregnant women with severe pre-eclampsia and normal pregnant women is related to the incidence of severe pre-eclampsia.
Total RNA was extracted from collected peripheral blood mononuclear cells from 20 or over weeks pregnant women diagnosed with severe pre-eclampsia (age: 31.57 ± 4.94) and normal pregnant women (age: 29.75 ± 4.6) respectively, followed by real-time PCR to examine the expression of let-7 s. Correlation between let-7 s expression level and maternal age or body mass index of the normal pregnant women were also analyzed using SPSS21.0 software.
Let-7a and let-7 g were significantly increased in pregnant women with severe pre-eclampsia by 4.67 fold and 2.37 fold respectively compared to the normal pregnant women, whereas there was no significant difference in let-7b and let-7i. Moreover, there was no correlation between maternal age or body mass index and the expression level of let-7a, let-7b, let-7 g, and let-7i.
In conclusion, let-7a and let-7 g were significantly increased in the PBMCs of severe pre-eclampsia women compared to normal controls. Moreover, their expression level was not correlated to the maternal age or body mass of patients. Our data indicated that let-7a and let-7 g may be considered as predictive markers for SPE.
目的：我们旨在研究严重子痫前期孕妇外周血血清中 let-7s 的表达水平与严重子痫前期的发生率之间是否存在相关性。
分别从诊断为严重子痫前期的 20 周以上孕妇（年龄：31.57±4.94）和正常孕妇（年龄：29.75±4.6）的外周血单个核细胞中提取总 RNA，然后进行实时 PCR 检测 let-7s 的表达。采用 SPSS21.0 软件分析 let-7s 表达水平与正常孕妇的母体年龄或体重指数之间的相关性。
与正常孕妇相比，严重子痫前期孕妇的 let-7a 和 let-7g 分别显著增加了 4.67 倍和 2.37 倍，而 let-7b 和 let-7i 则没有显著差异。此外，母体年龄或体重指数与 let-7a、let-7b、let-7g 和 let-7i 的表达水平之间没有相关性。
总之，与正常对照组相比，严重子痫前期孕妇的 PBMCs 中 let-7a 和 let-7g 的表达显著增加。此外，它们的表达水平与患者的母体年龄或体重无关。我们的数据表明，let-7a 和 let-7g 可以作为 SPE 的预测标志物。
