Land Academy for National Development (LAND), Zhejiang University, Hangzhou, 310058, China; Land Ecological Restoration Engineering Technology Research Center of Shandong Province, Binzhou, 256600, China.
Land Academy for National Development (LAND), Zhejiang University, Hangzhou, 310058, China; Land Ecological Restoration Engineering Technology Research Center of Shandong Province, Binzhou, 256600, China.
Ecotoxicol Environ Saf. 2019 Sep 30;180:557-564. doi: 10.1016/j.ecoenv.2019.05.057. Epub 2019 May 23.
As a global pollution problem, heavy metal contamination poses a serious hazard to soil microorganisms which play an extremely important role in soil chemical cycling and ecological persistence. However, the effects that different levels of heavy metal contamination in soils have on microorganisms and the interactions between them are still unclear. The purpose of this research is to analyze the microbial structure under different levels of heavy metal contamination, find out heavy metal tolerant species under different environmental conditions, then provide useful reference for the bioremediation of contaminated farmland. In this study, 16s rRNA high-throughput sequencing technology was used to investigate the microbial communities in severe level (SL), moderate level (ML), light level (LL) and clean level (CL) of heavy metal contaminated soils, and the relationships between environment variables and microorganisms were analyzed. The results showed that the concentrations of heavy metals and soil physicochemical properties had various impacts on microbial community composition under different heavy metal contamination levels. Most dominant bacteria were in significant negative correlation with Cd in ML region, and significantly correlated with TN and OM in LL region. However, there was no significant correlation between dominant fungi and the physicochemical properties in LL region. And most of the dominant fungi were significantly correlated with the heavy metal concentrations in SL region. The bacterial phyla such as Proteobacteria, Acidobacteria and Bacteroidetes showed more tolerance with heavy metal contamination in SL, ML and LL regions, respectively. Meanwhile, the dominant fungi of Ascomycota, Basidiomycota, Chytridiomycota, Glomeromycota, Zygomycota and Rozellomycota showed stronger correlations with heavy metal contamination in SL and LL regions. These results indicated that some microorganisms had strong tolerance to heavy metal contamination and had certain heavy metals digestion ability, which can create an appropriate soil environment for the growth of food crops.
作为一个全球性的污染问题，重金属污染对土壤微生物构成了严重的危害，而土壤微生物在土壤化学循环和生态持久力方面发挥着极其重要的作用。然而，不同程度的土壤重金属污染对微生物及其相互作用的影响仍不清楚。本研究旨在分析不同重金属污染水平下的微生物结构，找出不同环境条件下具有重金属耐受性的物种，为受污染农田的生物修复提供有用的参考。在这项研究中，我们使用 16s rRNA 高通量测序技术调查了严重污染（SL）、中度污染（ML）、轻度污染（LL）和清洁水平（CL）的重金属污染土壤中的微生物群落，并分析了环境变量与微生物之间的关系。结果表明，重金属浓度和土壤理化性质在不同重金属污染水平下对微生物群落组成有不同的影响。大多数优势细菌与 ML 区的 Cd 呈显著负相关，与 LL 区的 TN 和 OM 呈显著正相关。然而，在 LL 区，优势真菌与理化性质之间没有显著的相关性。大多数优势真菌与 SL 区的重金属浓度呈显著相关。在 SL、ML 和 LL 区，变形菌门、酸杆菌门和拟杆菌门等细菌门对重金属污染的耐受性更强。同时，子囊菌门、担子菌门、壶菌门、Glomeromycota、Zygomycota 和 Rozellomycota 等优势真菌与 SL 和 LL 区的重金属污染有更强的相关性。这些结果表明，一些微生物对重金属污染具有较强的耐受性，并且具有一定的重金属消化能力，可以为食用作物的生长创造适当的土壤环境。
