National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Sci Total Environ. 2019 Sep 10;682:532-540. doi: 10.1016/j.scitotenv.2019.05.197. Epub 2019 May 17.
As a solution of the sludge loss in the treatment of saline wastewater, the granulation of halophilic sludge was explored in this study. The inoculated estuarine sediment was granulated to an average diameter of 1155 ± 102 μm under the selective settling pressure in the airlift sequencing batch reactor (SBR) when the influent organic loading rate (OLR) was doubled to 0.36 g COD/L·day. The results indicated that the OLR doubled the amount of total extracellular polymeric substance (EPS) and that protein was predominant in the EPS (72.8 ± 2.0%). The correlation between aggregate size and protein content was better than that between aggregate size and polysaccharide content. The amount of alginate-like exopolysaccharides (ALE) increased linearly at the mature granular stage, co-occurring with the compact and elastic structure of the granules. According to the results of 16S rRNA high -throughput sequencing, the Shannon-Weaver index of mature granule decreased by >50% compared to the inoculated sediment. Bacteria of Propionibacteriaceae family constituted 34% of the population in granules and were in symbiotic relationship with halophiles of family Rhodocyclaceae, Vibrionaceae, Flavobacteriaceae, and Cryomorphaceae. The aerobic halophilic granular sludge showed COD removal efficiency of 90.9 ± 0.8% and ammonia removal efficiency of 72.6 ± 4.0% for 30 g/L saline wastewater. An average nitrite accumulation ratio of 94.5 ± 2.9% was observed during nitrification. Granulation of halophilic sludge provides an effective solution to the saline sludge loss problem, which is a step forward to realize the biological treatment of saline wastewater by halophiles.
为了解决含盐废水处理中污泥流失的问题，本研究探索了嗜盐污泥的颗粒化。在气升式序批式反应器（SBR）中，当进水有机负荷率（OLR）提高至 0.36 g COD/L·d 时，选择性沉降压力使接种河口沉积物颗粒化，平均粒径达到 1155±102μm。结果表明，OLR 使总胞外聚合物（EPS）的量增加了一倍，其中蛋白质是 EPS 的主要成分（72.8±2.0%）。颗粒大小与蛋白质含量的相关性优于颗粒大小与多糖含量的相关性。在成熟颗粒阶段，褐藻酸类胞外多糖（ALE）的量呈线性增加，与颗粒的紧密和弹性结构同时出现。根据 16S rRNA 高通量测序的结果，与接种沉积物相比，成熟颗粒的 Shannon-Weaver 指数下降了>50%。丙酸杆菌科的细菌在颗粒中占 34%，与 Rhodocyclaceae、Vibrionaceae、Flavobacteriaceae 和 Cryomorphaceae 家族的嗜盐菌共生。好氧嗜盐颗粒污泥对 30 g/L 盐水废水的 COD 去除率为 90.9±0.8%，氨氮去除率为 72.6±4.0%。硝化过程中平均亚硝酸盐积累率为 94.5±2.9%。嗜盐污泥的颗粒化提供了一种有效的解决含盐污泥流失问题的方法，这是实现嗜盐菌对含盐废水进行生物处理的重要一步。
