National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Chemosphere. 2021 Feb;264(Pt 1):128396. doi: 10.1016/j.chemosphere.2020.128396. Epub 2020 Sep 24.
Granulation of halophilic activated sludge is an important solution to solve the problem of solid-liquid separation in biological treatment of saline wastewater. This study demonstrated that by adding divalent cations into the saline influent with low organic load, halophilic granular sludge with an average diameter of 910 ± 10 μm can be cultivated. The close correlation between divalent cations and particle size indicated that Ca played a major role in the granulation process. Ca was accumulated in halophilic granular sludge, which provided an inorganic carrier for microbial aggregation and leaded to the dominance of halophilic bacteria of the family Flavobacteriaceae. The halophilic bacteria secreted a large amount of extracellular polymeric substances (EPS), which contained 70.0 ± 0.02% protein. By enhancing the EPS network of protein and Ca, halophilic granular sludge was formed. The addition of Mg enhanced the network of Mg and loosely bound EPS, which could be destroyed due to Na substitution. This study provides an effective granulation method for halophilic activated sludge.
嗜盐活性污泥的颗粒化是解决含盐废水生物处理中固液分离问题的重要方法。本研究表明，通过在低有机负荷的含盐进水 中添加二价阳离子，可以培养出平均直径为 910±10μm 的嗜盐颗粒污泥。二价阳离子与粒径之间的密切相关性表明 Ca 在颗粒化过程中起主要作用。Ca 在嗜盐颗粒污泥中积累，为微生物聚集提供了无机载体，导致嗜盐菌的优势属为黄杆菌科（Flavobacteriaceae）。嗜盐菌分泌大量的胞外聚合物物质（EPS），其中含有 70.0±0.02%的蛋白质。通过增强蛋白质和 Ca 的 EPS 网络，形成了嗜盐颗粒污泥。添加 Mg 增强了 Mg 和松散结合 EPS 的网络，但由于 Na 取代，这种网络可能会被破坏。本研究为嗜盐活性污泥的颗粒化提供了一种有效的方法。
